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Nitrate reductase involves in selenite reduction in Rahnella aquatilis HX2 and the characterization and anticancer activity of the biogenic selenium nanoparticles

文献类型： 外文期刊

作者： Li, Kui 1 ; Zhu, Yanyun 1 ; Zhang, Sasa 1 ; Xu, Qiaolin 1 ; Guo, Yanbin 1 ;

作者机构： 1.China Agr Univ, Coll Resources & Environm Sci, Beijing, Peoples R China

2.China Agr Univ, Beijing Key Lab Biodivers & Organ Farming, Beijing 100193, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing 210014, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China

关键词： Selenium nanoparticles; Biosynthesis; Nitrate reductase; Surface characteristics; Anticancer cell activity

期刊名称：JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0946-672X

年卷期： 2024 年 83 卷

页码：

收录情况： SCI

摘要： Background: Biogenic selenium nanoparticles (SeNPs) show numerous advantages including their high stability, low toxicity, and high bioactivity. While metabolism of SeNPs remains not well studied and need more investigation to reveal the process. Purpose: The objective of the study was to investigate the relationship between nitrate reductase and selenite reduction in Rahnella aquatilis HX2, characterize the properties of HX2 produced SeNPs, and explore their potential applications, particularly their anticancer activity. Procedures: Selenium species were measured by high-performance liquid chromatography coupled to inductively coupled plasma - Mass spectrometry (HPLC-ICP-MS). Transcription level of nitrate reductase was determined by Real-time quantitative PCR. Morphology, particle size, crystal structure and surface chemistry of SeNPs were determined by electron microscopy, dynamic light scattering method, Raman scattering, X-ray photoelectron spectroscopy, respectively. Anti cancer cell activity was measured by CCK-8 assay. Main findings: SeNP production in R. aquatilis HX2 was correlated with the cell growth. The products of selenite reduction in HX2 detected by HPLC-ICP-MS included SeNPs, selenocysteine (SeCys), Se-Methylselenocysteine (MeSeCys), and 7 unknown compounds. Nitrate addition experiments suggested the involvement of nitrate reductase in selenite reduction in HX2. Both the cellular membrane and cytoplasm of HX2 exhibited selenitereducing ability, indicating that membrane-associated nitrate reductase was not the sole selenite reductase in HX2. Characterization of the biogenic SeNPs revealed a spherical morphology and amorphous structure of them. Surface chemistry analysis implicated the binding of extracellular polymeric substances to the biogenic SeNPs, and the presence of Se0, Se2-, and electron-rich Se atoms on the surface of SeNPs. Finally, the IC50 values of the biogenic SeNPs were 36.49 mu M for HepG2 and 3.70 mu M for HeLa cells. Conclusions: The study first revealed that the nitrate reductase is involving in selenite reduction in R. aquatilis HX2. The biogenic SeNPs coordinated with organic substances in the surface. And SeNPs produced by R. aquatilis HX2 showed excellent anticancer activities on HepG2 and HeLa cells.

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