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The antibacterial mechanism of (-)-epigallocatechin-3-gallate (EGCG) against Campylobacter jejuni through transcriptome profiling

文献类型： 外文期刊

作者： Lin, Yilin 1 ; Liang, Siwei 1 ; Zhang, Yehui 2 ; Yu, Yigang 1 ;

作者机构： 1.South China Univ Technol, Res Ctr Food Safety & Detect, Sch Food Sci & Engn, Guangzhou, Peoples R China

2.Minist Agr & Rural Affairs, Sericulture & Agrifood Res Inst Guangdong Acad Agr, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc, Guangzhou, Peoples R China

3.Sericulture & Agrifood Res Inst Guangdong Acad Agr, Guangzhou, Peoples R China

关键词： antibacterial mechanism; campylobacter jejuni; (-)-Epigallocatechin-3-gallate; iron deficiency; motility; RNA-sequencing

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.9； 五年影响因子：4.0 ）

ISSN： 0022-1147

年卷期： 2024 年 89 卷 4 期

页码：

收录情况： SCI

摘要： (-)-Epigallocatechin-3-gallate (EGCG) has been shown antibacterial activity against Campylobacter jejuni; however, the relevant antibacterial mechanism is unknown. In this study, phenotypic experiments and RNA sequencing were used to explore the antibacterial mechanism. The minimum inhibitory concentration of EGCG on C. jejuni was 32 mu g/mL. EGCG-treated was able to increase intracellular reactive oxygen species levels and decline bacterial motility. The morphology and cell membrane of C. jejuni after EGCG treatment were observed collapsed, broken, and agglomerated by field emission scanning electron microscopy and fluorescent microscopy. The RNA-seq analysis presents that there are 36 and 72 differential expressed genes after C. jejuni was treated by EGCG with the concentration of 16 and 32 mu g/mL, respectively. EGCG-treated increased the thioredoxin expression, which was a critical protein to resist oxidative stress. Moreover, downregulation of the flgH and flgM gene in flagellin biosynthesis of C. jejuni was able to impair the flagella, reducing cell motility and virulence. The primary antibacterial mechanism revealed by RNA-seq is that EGCG with iron-chelating activity competes with C. jejuni for iron, causing iron deficiency in C. jejuni, which potentially impacts the survival and virulence of C. jejuni. The results suggested a new direction for exploring the activity of EGCG against C. jejuni in the food industry.

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