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Isolation and characterization of polymorphic microsatellite loci from a repeat-enriched genomic library of stone flounder (Kareius bicoloratus) and cross-species amplification

文献类型: 外文期刊

作者: Tian, Yong-Sheng 1 ; Miao, Gui-Dong 1 ; Shao, Chang-Wei 1 ; Liao, Xiao-Lin 1 ; Chen, Song-Lin 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Minist Agr, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

关键词: Stone flounder;Kareius bicoloratus;Microsatellite;Cross-species amplification

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

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收录情况: SCI

摘要: Stone flounder (Kareius bicoloratus) is a rare fish species in China. Here, we reported 11 polymorphic microsatellite loci isolated from a repeat-enriched genomic library of stone flounder. The number of alleles, observed and expected heterozygosity per locus in 32 individuals ranged from 3 to 6, from 0.1613 to 0.8667 and from 0.1549 to 0.7932, respectively. Two loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be useful for investigating genetic population structure and construction of genetic linkage map in Kareius bicoloratus.

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