microRNA-430b targets scavenger receptor class B member 1 (scarb1) and inhibits coloration and carotenoid synthesis in koi carp (Cyprinus carpio L.)
文献类型： 外文期刊
作者： Tian, Xue 1 ; Nian-nian Peng 1 ; Ma, Xiao 1 ; Li-min Wu 1 ; Shi, Xi 1 ; Hui-fen Liu 1 ; Hong-mei Song 3 ; Qi-sheng Wu 4 ; Xiao-lin Meng 1 ; Xue-jun Li 1 ;
作者机构： 1.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China
2.Henan Normal Univ, Engn Technol Res Ctr Henan Prov Aquat Anim Cultiv, Xinxiang 453007, Henan, Peoples R China
3.Minist Agr, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Chinese Acad Fishery Sci, Guangzhou 510380, Peoples R China
4.Fisheries Res Inst Fujian, Xiamen 361000, Peoples R China
关键词： miR-430; scarb1; Carotenoid metabolism; Skin color; Koi carp
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 546 卷
页码：
收录情况： SCI
摘要： In teleost fishes, miR-430 has several paralogs and is widely required for mesoderm formation and gonad development. However, the functions of miR-430 in skin pigmentation have not been completely elucidated. To understand the functions of miR-430 in color formation, we analyzed the conserved characteristics of miR-430 family in teleost, detected miR-430 family spatial and temporal expression patterns, verified the target differences between miR-430a and miR-430b, identified the phenotypic changes by overexpressing miR-430b in vivo and assessed the roles of miR-430b in coloration by analyzing the genes associated with pteridine and carotenoid pigment metabolism. The results showed that miR-430a and miR-430b were highly conserved in teleosts and that miR-430b was intensively expressed in skin, fins and scales comparing with miR-430a. Target verification analysis showed that miR-430b displayed significant repressive effects on scarb1 compared with miR-430a. In addition, overexpression of miR-430b led to diminished red intensity by decreasing skin carotenoid content and xanthophores/erythrophores density in scales. Accompanying the color dilution, the mRNA and protein levels of scarb1 were markedly inhibited by increased miR-430b, and several genes correlated with carotenoid metabolism also underwent corresponding modification. However, the contents of pteridine pigments and related genes experienced only limited changes. These findings suggest that miR-430b can improve skin pigmentation by blocking the expression of scarb1, resulting in carotenoid metabolism dysregulation and chromatophores density reduction.
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