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Female-specific SNP markers provide insights into a WZ/ZZ sex determination system for mud crabs Scylla paramamosain, S-tranquebarica and S-serrata with a rapid method for genetic sex identification

文献类型： 外文期刊

作者： Shi, Xi 1 ; Waiho, Khor 1 ; Li, Xincang 4 ; Ikhwanuddin, Mhd 5 ; Miao, Guidong 1 ; Lin, Fan 1 ; Zhang, Yueling 1 ; Li, Sh 1 ;

作者机构： 1.Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, 243 Daxue Rd, Shantou 515063, Peoples R China

2.Shantou Univ, STU UMT Joint Shellfish Res Lab, Shantou 515063, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

5.Univ Malaysia Terengganu, Inst Trop Aquaculture, Kuala Terengganu 21030, Malaysia

6.Univ Philippines, Div Biol Sci, Coll Arts & Sci, Visayas 5023, Miagao, Philippines

7.Southeast Asian Fisheries Dev Ctr, Dept Aquaculture, Tigbauan 5021, Philippines

关键词： Scylla spp; RAD-seq; Female-specific SNP markers; WZ; ZZ sex determination system; Genetic sex identification

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2018 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundMud crabs, Scylla spp., are commercially important large-size marine crustaceans in the Indo-West Pacific region. As females have the higher growth rate and economic value, the production of all female stocks is extremely essential in aquaculture. However, the sex determination mechanism is still unclear. Development of sex-specific genetic markers based on next-generation sequencing proved to be an effective tool for discovering sex determination system in various animals.ResultsRestriction-site associated DNA sequencing (RAD-seq) was employed to isolate sex-specific SNP markers for S. paramamosain. A total of 335.6 million raw reads were obtained from 20 individuals, of which 204.7 million were from 10 females and 130.9 million from 10 males. After sequence assembly and female-male comparison, 20 SNP markers were identified to be sex-specific. Furthermore, ten SNPs in a short sequence (285bp) were confirmed heterozygous in females and homozygous in males in a large population by PCR amplification and sequencing. Subsequently, a female-specific primer was successfully designed according to the female-specific nucleotide which could amplify an expected band from females but not from males. Thus, a rapid and effective method for molecular sexing in S. paramamosain was developed, meanwhile, this method could successfully identify the sex of S. tranquebarica and S. serrata. Finally, nine and four female-specific SNP markers were detected in S. tranquebarica and S. serrata, respectively.ConclusionsSex-specific SNP markers were firstly identified in crab species and showed female heterogamety and male homogamety, which provided strong genetic evidence for a WZ/ZZ sex determination system in mud crabs S. paramamosain, S. tranquebarica and S. serrata. These findings will lay a solid foundation for the study of sex determination mechanism, sex chromosome evolution, and the development of mono-sex population in crustaceans.

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