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Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08

文献类型: 外文期刊

作者: Sun, Haiyan 1 ; Zhao, Pingjuan 1 ; Peng, Ming 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, State Key Lab Trop Corp Biotechnol, Haikou 571101, Peoples R China

期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )

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收录情况: SCI

摘要: The effect of the addition of maltitol on the production of raw starch digesting glucoamylase (RSDG) by Aspergillus niger F-08 was studied in the paper. The activity of RSDG formed by Aspergillus niger F-08 was enhanced dramatically by the addition of maltitol to the medium and it was confirmed that maltitol acted as a very efficient inducer for RSDG production. As a result, with the addition of 8 g maltitol in 1 l basal medium at the beginning of the culture, the enzyme activity was enhanced near 8-fold. The synthesis mechanism of RSDG by Aspergillus niger F-08 was also studied on molecular level. It was found that the regulation of RSDG synthesis by Aspergillus niger F-08 occurred at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of maltitol at transcriptional level.

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