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Biotransformation of gallate esters by a pH-stable tannase of mangrove-derived yeast Debaryomyces hansenii

文献类型： 外文期刊

作者： Song, Lei 1 ; Wang, Xiao-Chen 2 ; Feng, Zhen-Quan 3 ; Guo, Yan-Feng 1 ; Meng, Guo-Qing 1 ; Wang, Hai-Ying 4 ;

作者机构： 1.Heze Univ, Coll Agr & Bioengn, Heze, Peoples R China

2.Univ Hlth & Rehabil Sci, Qingdao Municipal Hosp, Dept Clin Lab, Qingdao, Shandong, Peoples R China

3.Linyi Vocat Univ Sci & Technol, Coll Ecol & Environm Protect, Linyi, Peoples R China

4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao, Peoples R China

关键词： tannase; pH stability; tannin biodegradation; gallate esters; gallic acid

期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： Introduction: Tannase is a crucial enzyme that finds wide applications in the pharmaceutical industry, feed processing, and beverage manufacturing. Although extensive studies have been conducted on tannases from fungi and bacteria, reports on tannases exhibiting favorable pH stability are relatively limited. Methods: In this study, a tannin-degrading strain Debaryomyces hansenii was screened to induce tannase production, and the corresponding tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. SDS-PAGE analysis revealed that the purified TanF tannase had a molecular weight of approximately 70 kDa.Results and Discussion: The enzyme demonstrated optimal activity at 40 degrees C and retained over 80% of its activity in the range of 35 degrees C-60 degrees C. Of particular interest, TanF exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0-8.0. Furthermore, TanF exhibited broad substrate specificity for gallate esters. The final gallic acid production by TanF from tannic acid achieved 18.32 g/L. Therefore, the excellent properties TanF has been demonstrated to be an efficient tool for the preparation of gallic acid.

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