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Development of polymorphic microsatellite markers from barfin flounder (Verasper moseri) and their cross-species amplification

文献类型: 外文期刊

作者: Miao, Gui-Dong 1 ; Shao, Chang-Wei 1 ; Liao, Xiao-Lin 1 ; Ma, Hong-Yu 1 ; Tian, Yong-Sheng 1 ; Chen, Song-Lin 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

关键词: Barfin flounder;Verasper moseri;Microsatellites;SSR

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Barfin flounder (Verasper moseri) is a rare fish species in the world. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library of barfin flounder (Verasper moseri). The number of alleles, observed, and expected heterozygosity per locus in a test population ranged from 2 to 6, from 0.3333 to 1.0000, and from 0.4866 to 0.7774, respectively. One locus significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification of these microsatellite loci in additional five fish species was performed. These polymorphic microsatellite loci would be useful for investigating genetic population structure and construction of genetic linkage map in Verasper moseri.

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