您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Preparation and immunogenicity studies of NvIBDV VP2-ferritin nanoparticles

文献类型： 外文期刊

作者： Gao, Shimin 1 ; Zhou, Xinrui 1 ; Li, Xinya 1 ; Luo, Liezhu 1 ; Yang, Kun 1 ; Huang, Yunzhen 2 ; Bao, Yun 1 ; Wu, Xingchen 1 ; Guo, Yanna 1 ; Li, Junping 1 ; Shao, Yuanmeng 1 ; Wang, Longlong 1 ; Liu, Zhe 1 ; Sun, Minhua 2 ; Liang, Libin 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Vet Med, Jinzhong 030801, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China

关键词： Infectious bursal disease virus; Novel mutant strain; Ferritin; Nanoparticles; Subunit vaccine

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）

ISSN：

年卷期： 2025 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundInfectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is a highly contagious disease that is prevalent worldwide and poses a significant threat to the poultry industry. While commercially available vaccines are used for prevention, IBD outbreaks remain frequent.ObjectiveThe continuous mutation of virulent strains and their ability to evade traditional vaccine protection complicate IBD control, which necessitates the development of novel vaccines and a deeper understanding of viral mutation mechanisms.MethodUtilizing the self-assembly capability of ferritin (Fe), the hypervariable region (HVR) protein of a novel variant IBDV (NvIBDV) VP2 was displayed on the ferritin shell, forming regular nanoparticles. The full-length NvIBDV VP2 protein and the NvIBDV VP2-HVR-Fe fusion protein were prokaryotically expressed in E. coli and purified to prepare a VP2 protein vaccine and a VP2-Fe nanoparticle vaccine. An inactivated NvIBDV vaccine served as a control for evaluating immunogenicity and protection.ResultsRecombinant prokaryotic expression vectors pET-VP2-Fe (encoding VP2-HVR-Fe) and pET-VP2 (encoding full-length VP2) were successfully constructed. Soluble VP2-Fe and VP2 proteins were expressed and purified. Electron microscopy confirmed the formation of a cage-like nanoparticle structure for VP2-Fe. Immunization of SPF chickens with NvIBDV VP2-Fe nanoparticles induced a robust immune response characterized by high antibody titers and a significantly high protection rate against viral challenge.ConclusionThe successfully constructed recombinant subunit nanoparticle vaccine, which displays the NvIBDV VP2 HVR on ferritin, effectively increased the antibody titer and provided superior immune protection. This approach offers a feasible strategy for developing novel IBDV subunit vaccines.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台