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An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus

文献类型： 外文期刊

作者： Wang, Jing 1 ; Wang, Qing 1 ; Huang, Pengyun 1 ; Qu, Yingmin 2 ; Huang, Zhicheng 1 ; Wang, Huan 1 ; Liu, Xiao-Hong 3 ; Lin, Fu-Cheng 2 ; Lu, Jianping 1 ;

作者机构： 1.Zhejiang Univ, Coll Life Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

3.Zhejiang Univ, Inst Biotechnol, Hangzhou, Peoples R China

关键词： Magnaporthe oryzae; appressorium formation; endosome; cAMP; pathogenicity; cell death; cAMP-PKA; endolysosome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： The rice blast fungus Magnaporthe oryzae spores differentiate and mature into functional appressoria by sensing the host surface signals. Environmental stimuli are transduced into cells through internalization during appressorium formation, such as in the cAMP-PKA pathway. Here, we describe a novel contribution to how appressoria mature on the surface of a leaf, and its connection to endosomes and the cAMP-PKA pathway. An appressorium membrane-specific protein, Pams1, is required for maintaining endosomal structure, appressorium maturation, and virulence in M. oryzae. During appressorium development, Pams1 was translocated from the cell membrane to the endosomal membrane. Deletion of PAMS1 led to the formation of two types of abnormal appressoria after 8 h post inoculation (hpi): melanized type I had a reduced virulence, while pale type II was dead. Before 8 hpi, Delta pams1 formed appressoria that were similar to those of the wild type. After 8 hpi, the appressoria of Delta pams1 was differentiated into two types: (1) the cell walls of type I appressoria were melanized, endosomes were larger, and had a different distribution from the wild type and (2) Type II appressoria gradually stopped melanization and began to die. The organelles, including the nucleus, endosomes, mitochondria, and endoplasmic reticula, were degraded, leaving only autophagic body-like vesicles in type II appressoria. The addition of exogenous cAMP to Delta pams1 led to the formation of a greater proportion of type I appressoria and a smaller proportion of type II appressoria. Thus, defects in endosomal structure and the cAMP-PKA pathway are among the causes of the defective appressorium maturation and virulence of Delta pams1.

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