Isolation and characterization of 12 polymorphic microsatellite markers from ladyfish (Elops saurus Linnaeus)
文献类型: 外文期刊
作者: Sha, Zhen-Xia 1 ; Xing, Shi-Chao 1 ; Shao, Chang-Wei 1 ; Tian, Yong-Sheng 1 ; Liao, Xiao-Lin 1 ; Chen, Song-Lin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
关键词: Ladyfish;Elops saurus Linnaeus;Enriched genomic library;SSR;Microsatellites
期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )
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收录情况: SCI
摘要: Ladyfish (Elops saurus Linnaeus) is an economically important marine fish species. 76 microsatellite loci were isolated from an enriched genomic library of Elops saurus. Twelve of these markers were polymorphic in a test population with alleles per locus ranging from three to nine. The number of observed, expected heterozygosity and polymorphism information content (PIC) per locus in 20 individuals ranged from 0.2000 to 1.0000, 0.1897-0.8846, 0.1769-0.8476, respectively. One markers significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of markers. As a result, 12 microsatellite markers probably should provide sufficient level of genetic diversity to investigate the fine-scale population structure, stock management and enhancement, genetic linkage map construction and molecular marker-assisted breeding in Elops saurus Linnaeus.
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