您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide association study and development of molecular markers for yield and quality traits in peanut (Arachis hypogaea L.)

文献类型： 外文期刊

作者： Guo, Minjie 1 ; Deng, Li 1 ; Gu, Jianzhong 1 ; Miao, Jianli 1 ; Yin, Junhua 1 ; Li, Yang 1 ; Fang, Yuanjin 2 ; Huang, Bingyan 2 ; Sun, Ziqi 2 ; Qi, Feiyan 2 ; Dong, Wenzhao 2 ; Lu, Zhenhua 1 ; Li, Shaowei 1 ; Hu, Junping 1 ; Zhang, Xinyou 2 ; Ren, Li 1 ;

作者机构： 1.Kaifeng Acad Agr & Forestry Sci, Peanut Inst, Kaifeng 475004, Peoples R China

2.Henan Acad Agr Sci, Henan Acad Crop Mol Breeding, Shennong Lab, Henan Prov Key Lab Oil Crops Improvement, Zhengzhou 450002, Peoples R China

关键词： Peanut; Yield traits; Quality traits; Re-sequencing; Genome-wide association study (GWAS); Kompetitive allele-specific PCR (KASP) marker

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background This study aims to decipher the genetic basis governing yield components and quality attributes of peanuts, a critical aspect for advancing molecular breeding techniques. Integrating genotype re-sequencing and phenotypic evaluations of seven yield components and two grain quality traits across four distinct environments allowed for the execution of a genome-wide association study (GWAS). Results The nine phenotypic traits were all continuous and followed a normal distribution. The broad heritability ranged from 88.09 to 98.08%, and the genotype-environment interaction effects were all significant. There was a highly significant negative correlation between protein content (PC) and oil content (OC). The 10x genome re-sequencing of 199 peanut accessions yielded a total of 631,988 high-quality single nucleotide polymorphisms (SNPs), with 374 significant SNP loci identified in association with the nine traits of interest. Notably, 66 of these pertinent SNPs were detected in multiple environments, and 48 of them were linked to multiple traits of interest. Five loci situated on chromosome 16 (Chr16) exhibited pleiotropic effects on yield traits, accounting for 17.64-32.61% of the observed phenotypic variation. Two loci on Chr08 were found to be strongly associated with protein and oil contents, accounting for 12.86% and 14.06% of their respective phenotypic variations, respectively. Linkage disequilibrium (LD) block analysis of these seven loci unraveled five nonsynonymous variants, leading to the identification of one yield-related candidate gene and two quality-related candidate genes. The correlation between phenotypic variation and SNP loci in these candidate genes was validated by Kompetitive allele-specific PCR (KASP) marker analysis. Conclusions Overall, molecular markers were developed for genetic loci associated with yield and quality traits through a GWAS investigation of 199 peanut accessions across four distinct environments. These molecular tools can aid in the development of desirable peanut germplasm with an equilibrium of yield and quality through marker-assisted breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台