您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris

文献类型： 外文期刊

作者： Wang, Sanhong 1 ; Yao, Quanhong 2 ; Tao, Jianmin 2 ; Qiao, Yushan 2 ; Zhang, Zhen 2 ;

作者机构： 1.Nanjing Agr Univ, Dept Hort, Lab Fruit Tree Mol Breeding, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Dept Hort, Lab Fruit Tree Mol Breeding, Nanjing 210095, Peoples R China; Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

关键词： glycine betaine synthesis genes;2A region of foot-and-mouth disease virus;coexpression;stress tolerance;Pichia pastoris;MOUTH-DISEASE VIRUS;ALDEHYDE DEHYDROGENASE;TRANSGENIC PLANTS;ESCHERICHIA-COLI;MULTIPLE GENES;CLEAVAGE;TOLERANCE;CHOLINE;POLYPROTEIN;STRESS

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The genes encoding the two enzymes choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH) of glycine betaine synthesis in Suaeda salsa were cloned and fused with the 2A region of foot-and-mouth disease virus in a single open reading frame. The fused genes were placed under the control of the alcohol oxidase (AOX1) promoter in pPIC3B and transformed into P. pastoris GS115. The expression of the fused genes in P. pastoris and the ability of recombinant yeasts to tolerate environmental stresses were studied. The results showed that induced with 0.5% methanol for 96 h, the maximal activities of CMO and BADH in the tested recombinant yeasts were 45- and 44-fold higher than those in the control yeast transformed empty vector only, respectively; the content of glycine betaine in the recombinant yeasts was 28- to 35-fold higher than that in the control. The fused genes linked by 2A region of foot-and-mouth disease virus were expressed in P. pastoris successfully and the polyprotein was 'cleaved' to each functional protein. The yeasts transformed the fused genes, which were more resistant to salt, methanol, and high temperature stresses than the control as result of glycine betaine synthesis genes introduced.

  • 相关文献

[1]Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Li, Xian,Xue, Yong,Liu, Jin-Ge,Gao, Feng,Cai, Bin,Chen, Jian-Min,Yao, Quan-Hong.

[2]Agrobacterium-mediated multiple gene transformation in rice using a single vector. Cao, MX,Huang, JQ,Wei, ZM,Yao, QH,Wan, CZ,Lu, JA. 2005

[3]Near-infrared reflectance Spectroscopy-based methods for phytase registration in feed industry. Yang, Haifeng,Lv, Xiaowen,Wang, Jing,Li, Junguo,Li, Hui,Qin, Yuchang.

[4]A semi-rational design strategy of directed evolution combined with chemical synthesis of DNA sequences. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Gao, Feng,Xu, Fang,Cai, Bin,Yao, Quan-Hong.

[5]High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris. Xiong, Ai-Sheng,Yao, Quan-Hong,Peng, Ri-He,Zhang, Zhen,Xu, Fang,Liu, Jin-Ge,Han, Pei-Lai,Chen, Jian-Min.

[6]A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity. Peng, R,Xiong, A,Li, X,Fuan, H,Yao, Q.

[7]AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis. Xu, Jing,Tian, Yong-Sheng,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Jin, Xiao-Fen,Gao, Feng,Fu, Xiao-Yan,Yao, Quan-Hong,Xu, Jing,Hou, Xi-Lin. 2010

[8]Priming of rice (Oryza sativa L.) seedlings with abscisic acid enhances seedling survival, plant growth, and grain yield in saline-alkaline paddy fields. Wei, Li-Xing,Lv, Bing-Sheng,Li, Xiao-Wei,Wang, Ming-Ming,Ma, Hong-Yuan,Yang, Hao-Yu,Liang, Zheng-Wei,Ma, Hong-Yuan,Yang, Hao-Yu,Yang, Rui-Fang,Liang, Zheng-Wei,Liang, Zheng-Wei,Wei, Li-Xing,Wang, Zhi-Hua,Lou, Jin-Hua,Lv, Bing-Sheng,Yang, Rui-Fang,Piao, Zhong-Ze,Jiang, Chang-Jie.

[9]Isolation and characterization of a harvest-induced promoter of an alfalfa gene, hi7. Zhang, Jian,Yu, Wen-Jin,Bahramnejad, Bahman,Erickson, Larry R.,Xiong, Ai-Sheng,Bahramnejad, Bahman,Yu, Wen-Jin,Erickson, Larry R..

[10]Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance. Xue, Y.,Peng, R.,Xiong, A.,Li, X.,Yao, Q.,Zha, D.. 2010

[11]Transcription factor OsAP21 gene increases salt/drought tolerance in transgenic Arabidopsis thaliana. Jin, Xiaofeng,Xue, Yong,Xu, RanRan,Bian, Lin,Zhu, Bo,Han, Hongjuan,Peng, Rihe,Yao, Quanhong,Wang, Ren.

[12]Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza sativa L.. Qin, Qiu-lin,Liu, Jin-ge,Zhang, Zhen,Peng, Ri-he,Xiong, Ai-sheng,Yao, Quan-hong,Chen, Jian-min.

[13]Yeast copper-dependent transcription factor ACE1 enhanced copper stress tolerance in Arabidopsis. Xu, Jing,Tian, Yong-Sheng,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Jin, Xiao-Fen,Gao, Jian-Jie,Yao, Quan-Hong,Xu, Jing,Gao, Jian-Jie,Hou, Xi-Lin.

[14]Expression of foot-and-mouth disease virus epitopes in tobacco by a tobacco mosaic virus-based vector. Wu, LG,Jiang, LB,Zhou, ZA,Fan, JH,Zhang, QQ,Zhu, HH,Han, Q,Xu, ZK.

[15]Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis. Zhang, Yang,Chen, Chen,Jin, Xiao-Fen,Xiong, Ai-Sheng,Peng, Ri-He,Yao, Quan-Hong,Zhang, Yang,Chen, Chen,Hong, Yi-Huan,Chen, Jian-Min.

[16]Invasive ornamental plants: Problems, challenges, and molecular tools to neutralize their invasiveness. Li, Y,Cheng, ZM,Smith, WA,Ellis, DR,Chen, YQ,Zheng, XL,Pei, Y,Luo, KM,Zhao, DG,Yao, QH,Duan, H,Li, Q.

[17]Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis. Xue, Yong,Peng, Rihe,Xiong, Aisheng,Li, Xian,Yao, Quanhong,Zha, Dingshi.

[18]A Vitis vinifera xanthine dehydrogenase gene, VvXDH, enhances salinity tolerance in transgenic Arabidopsis. You, Shuanghong,Sun, Miao,Zhu, Bo,Wang, Feibing,Han, Hongjuan,Zhu, Hengweng,Peng, Rihe,Yao, Quanhong.

[19]Expression analysis of three SERK-like genes in barley under abiotic and biotic stresses. Li, Yingbo,Liu, Chenghong,Guo, Guimei,He, Ting,Chen, Zhiwei,Gao, Runhong,Xu, Hongwei,Lu, Ruiju,Huang, Jianhua,Faheem, Muhammad. 2017

[20]Isolation of a choline monooxygenase cDNA clone from Amaranthus tricolor and its expressions under stress conditions. Meng, YL,Wang, YM,Zhang, DB,Nii, N.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台