文献类型： 外文期刊
作者： Guo, Xiaoli 1 ; Zhao, Chengcheng 4 ; Yang, Ruifei 4 ; Wang, Yuzhe 4 ; Hu, Xiaoxiang 4 ;
作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510640, Peoples R China
2.Guangdong Acad Agr Sci, Guangdong Prov Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China
4.China Agr Univ, Coll Biol Sci, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China
关键词： ABCD4; HC11; Mammary gland development; PRLR
期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）
ISSN： 1471-2164
年卷期： 2024 年 25 卷 1 期
页码：
收录情况： SCI
摘要： Background Mammary gland development is a critical process in mammals, crucial for their reproductive success and offspring nourishment. However, the functional roles of key candidate genes associated with teat number, including ABCD4, VRTN, PROX2, and DLST, in this developmental process remain elusive. To address this gap in knowledge, we conducted an in-depth investigation into the dynamic expression patterns, functional implications, and regulatory networks of these candidate genes during mouse mammary gland development. Results In this study, the spatial and temporal patterns of key genes were characterized in mammary gland development. Using time-series single-cell data, we uncovered differences in the expression of A bcd4, Vrtn, Prox2, and Dlst in cell population of the mammary gland during embryonic and adult stages, while Vrtn was not detected in any cells. We found that only overexpression and knockdown of Abcd4 could inhibit proliferation and promote apoptosis of HC11 mammary epithelial cells, whereas Prox2 and Dlst had no significant effect on these cells. Using RNA-seq and qPCR, further analysis revealed that Abcd4 can induce widespread changes in the expression levels of genes involved in mammary gland development, such as Igfbp3, Ccl5, Tlr2, and Prlr, which were primarily associated with the MAPK, JAK-STAT, and PI3K-AKT pathways by functional enrichment. Conclusions These findings revealed ABCD4 as a candidate gene pivotal for regulating mammary gland development and lactation during pregnancy by influencing PRLR expression.
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