Single-Cell Analysis of Microbial Degradation Mechanisms and Remediation Potential for Emerging Pollutants: A Case Study on Methylnaphthalene
文献类型： 外文期刊
作者： Li, Jibing 1 ; Cai, Xixi 3 ; Deng, Yirong 4 ; Zhang, Dayi 5 ; Li, Bei 7 ; Luo, Chunling 1 ; Zhang, Gan 1 ;
作者机构： 1.Chinese Acad Sci, State Key Lab Adv Environm Technol, Guangzhou 510640, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China
4.Guangdong Prov Acad Environm Sci, Guangdong Key Lab Contaminated Environm Management, Guangzhou 510045, Peoples R China
5.Jilin Univ, Minist Educ, Key Lab Groundwater Resources & Environm, Changchun 130012, Peoples R China
6.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China
7.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, State Key Lab Appl Opt, Changchun 130033, Peoples R China
关键词： stable-isotope probing; Raman-activated cell sorting; PAH derivatives; single-cell genomic sequencing; functional degraders
期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY （ 影响因子：11.3； 五年影响因子：12.4 ）
ISSN： 0013-936X
年卷期： 2025 年 59 卷 9 期
页码：
收录情况： SCI
摘要： Polycyclic aromatic hydrocarbon (PAH) derivatives, including alkyl-PAHs, are significant emerging environmental contaminants. Traditional culture techniques often fail to isolate functional microorganisms responsible for their in situ degradation, leaving their metabolic interactions and pathways largely uncharacterized. This study employs 2-methylnaphthalene (MP) as a model compound and utilizes Raman-activated cell sorting with stable isotope probing (RACS-SIP) and single-cell genome sequencing to investigate alkyl-PAH degradation in petroleum-contaminated wastewater. RACS-SIP identified two key in situ MP-degrading microbes, Sphingomonas sp. and Pseudomonas sp., showing Raman shifts from 1001 to 968 cm-1 and 782 to 768 cm-1. Genome analysis linked these microorganisms to their specific genes and metabolic pathways, revealing distinct degradation mechanisms: Sphingomonas sp. hydroxylates nonmethyl-substituted aromatic rings, while Pseudomonas sp. utilizes both hydroxylation and methyl end oxidation. These findings were substantiated by functional gene quantification, emphasizing the potential of microbial consortia with diverse metabolic pathways to enhance degradation efficiency through cooperative interactions and reduced metabolic load. Moreover, the successful cultivation of RACS-sorted bacteria demonstrated superior pollutant removal compared to traditional methods. This study advances our understanding of microbial biotransformation of emerging contaminants and demonstrates the necessity of precisely identifying and cultivating in situ functional microorganisms to develop robust consortia for sustainable pollutant degradation.
- 相关文献
作者其他论文 更多>>
-
Synergistic effects of surfactant biostimulation and indigenous fungal bioaugmentation for enhanced bioremediation of PAH-contaminated soils
作者：Dai, Yeliang;Wang, Shuang;Yang, Xiumin;Zhao, Xuan;Cheng, Xianghui;Li, Jibing;Luo, Chunling;Zhang, Gan;Dai, Yeliang;Zhao, Chunxia;Wang, Xiyu;Dai, Yeliang;Cai, Xixi;Wang, Shuang;Zhao, Xuan
关键词：Indigenous fungal bioaugmentation; Surfactant biostimulation; Polycyclic aromatic hydrocarbons; Indigenous functional microorganism; Stable-isotope probing
-
Influence of C/N ratio on biological denitrification in the context of electro-anammox
作者：Lin, Weisheng;Tang, Jiahuan;Qin, Hongjie;Cai, Xixi;Qin, Hongjie;Cai, Xixi;Zheng, Linlan
关键词：Anammox; Biological denitrification; Electroactive biofilm; Carbon-to-nitrogen ratio
-
Microbial consortium assembly and functional analysis via isotope labelling and single-cell manipulation of polycyclic aromatic hydrocarbon degraders
作者：Li, Jibing;Luo, Chunling;Guan, Guoqing;Zhang, Gan;Li, Jibing;Luo, Chunling;Guan, Guoqing;Zhang, Gan;Li, Jibing;Luo, Chunling;Guan, Guoqing;Zhang, Gan;Cai, Xixi;Zhang, Dayi;Zhang, Dayi;Li, Bei;Li, Bei
关键词：in situ functional microbial consortia; Raman-activated cell sorting; stable-isotope probing; genome-directed cultivation; soil phenanthrene-degrading bacteria; single-cell genomic sequencing
-
Using rice straw-augmented ecological floating beds to enhance nitrogen removal in carbon-limited wastewater
作者：Cai, Xixi;Li, Jianying;Wu, Haoping;Yang, Siyu;You, Yi;Zou, Chunping;Qin, Hongjie;Cai, Xixi;Li, Jianying;Wu, Haoping;Yang, Siyu;You, Yi;Zou, Chunping;Qin, Hongjie;Li, Dunhai;Xing, Wei;Guo, Xiaoyu;Li, Jibing;Li, Jibing
关键词：Microbial community; Denitrification; Low -carbon sewage; Aquatic macrophytes
-
Metabolic Characterization and Geochemical Drivers of Active Hydrocarbon-Degrading Microorganisms
作者：Li, Jibing;Huang, Qihui;Zhao, Xuan;Luo, Chunling;Zhang, Gan;Li, Jibing;Huang, Qihui;Zhao, Xuan;Luo, Chunling;Zhang, Gan;Li, Jibing;Luo, Chunling;Zhang, Gan;Huang, Qihui;Cai, Xixi;Zhao, Xuan
关键词：stable-isotope probing; metagenomic binning; functional microbial community; geochemical factor analysis; metabolic characteristics
-
Colloidal fraction on pomelo peel-derived biochar plays a dual role as electron shuttle and adsorbent in controlling arsenic transformation in anoxic paddy soil
作者：Zhong, Ruilin;Pan, Dandan;Huang, Guoyong;Yang, Guang;Wang, Xiaonan;Niu, Rumiao;Ding, Ziman;Chi, Wenting;Li, Xiaomin;Pan, Dandan;Huang, Guoyong;Chi, Wenting;Wang, Ying;Cai, Xixi
关键词：Biochar colloids; As(V) reduction; Electron transfer capability; As(III) immobilization; Adsorption
-
Unveiling the synergistic mechanism of autochthonous fungal bioaugmentation and ammonium nitrogen biostimulation for enhanced phenanthrene degradation in oil-contaminated soils
作者：Dai, Yeliang;Li, Jibing;Wang, Shuang;Zhao, Xuan;Cheng, Xianghui;Huang, Qihui;Yang, Xiumin;Luo, Chunling;Zhang, Gan;Dai, Yeliang;Li, Jibing;Wang, Shuang;Zhao, Xuan;Cheng, Xianghui;Huang, Qihui;Yang, Xiumin;Luo, Chunling;Zhang, Gan;Dai, Yeliang;Li, Jibing;Wang, Shuang;Zhao, Xuan;Cheng, Xianghui;Luo, Chunling;Zhang, Gan;Cai, Xixi;Huang, Qihui
关键词：Nitrogen biostimulation; PAHs; Autochthonous fungal bioaugmentation; Degrading microorganism; Stable-isotope-probing
