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RNase H2 triggered visual loop-mediated isothermal amplification combining smartphone assisted all-in-one aptamer magnetic enrichment device for ultrasensitive culture-independent detection of Salmonella Typhimurium in chicken meat

文献类型： 外文期刊

作者： Jia, Kaiyuan 1 ; Xiao, Renhang 1 ; Lin, Qijie 1 ; Gou, Hongchao 5 ; Peng, Junhao 1 ; Liang, Yucen 1 ; Shen, Haiyan 5 ; Cai, Mingrui 2 ; Liao, Ming 5 ; Zhang, Jianmin 1 ; Han, Yuxing 3 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Natl & Reg Joint Engn Lab Medicament Zoonoses Prev, Ctr Emerging & Zoonot Dis,Key Lab Zoonoses,Minist, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Coll Elect Engn, Coll Artificial Intelligence, Guangzhou 510642, Peoples R China

3.Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China

4.Tsinghua Univ, RIOS Lab, Shenzhen 518055, Peoples R China

5.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China

6.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

关键词： Culture -independent detection; Visual LAMP; Palm -held device; Magnetic enrichment

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2023 年 380 卷

页码：

收录情况： SCI

摘要： Pre-enrichment cultural continues to remain time-consuming and labor-intensive in the detection of lowconcentration bacteria in meat samples. Culture-independent detection method is urgently needed in POCT technology. In order to be able to proceed with culture-independent pre-enrichment for low-concentration pathogen detection, a RNase H2 enzyme triggered all-in-one device-based visual Loop-mediated Isothermal Amplification detection method was developed, combining with Salmonella Typhimurium specific Aptamer Magnetic Enrichment steps, termed as the AME-LAMP method. In order to better enable the AME-LAMP method to perform on-site culture-independent detection, a palm-held all-in-one device (PAD) was designed and manufactured. Further, a self-developed smart phone fluorescence analysis app could complete end-point detection, thus eliminating miscalculation. RNase H2 Enzyme triggered AME-LAMP method could detect Salmonella as low as 5.5 CFU/mL while conventional LAMP only detected 550 CFU/mL. By verifying the detection performance of AME-LAMP methods in chicken meat samples, AME-LAMP shows the potential to achieve ultrasensitive Salmonella detection by comparing with ISO isolation method and normal LAMP. With the assistance of smart phone-based PAD, it can be reliably applied for the on-site culture-independent detection of Salmonella Typhimurium.

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