您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Porcine extraintestinal pathogenic Escherichia coli delivers two serine protease autotransporters coordinately optimizing the bloodstream infection

文献类型： 外文期刊

作者： Pan, Xinming 1 ; Chen, Rong 1 ; Zhang, Yating 1 ; Zhu, Yinchu 4 ; Zhao, Jin 5 ; Yao, Huochun 1 ; Ma, Jiale 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Minist Educ MOE Joint Int Res Lab Anim Hlth & Food, Nanjing, Peoples R China

2.Minist Agr, Key Lab Anim Bacteriol, Nanjing, Peoples R China

3.Nanjing Agr Univ, Off Int Des OIE Reference Lab Swine Streptococcosi, Nanjing, Peoples R China

4.Inst Anim Husb & Vet Sci, Zhejiang Acad Agr Sci, Hangzhou, Peoples R China

5.Yuxi Agr Vocat Tech Coll, Dept Anim Sci, Yuxi, Peoples R China

关键词： extraintestinal pathogenic E; coli; bloodstream; serine protease autotransporter; immunomodulation; mucin-like glycoprotein

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.7； 五年影响因子：5.9 ）

ISSN： 2235-2988

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the leading causes of bloodstream infections in a broad spectrum of birds and mammals, thus poses a great threat to public health, while its underlying mechanism causing sepsis is not fully understood. Here we reported a high virulent ExPEC strain PU-1, which has a robust ability to colonize within host bloodstream, while induced a low level of leukocytic activation. Two serine protease autotransporters of Enterobacteriaceae (SPATEs), Vat(PU-1) and Tsh(PU-1), were found to play critical roles for the urgent blood infection of strain PU-1. Although the Vat and Tsh homologues have been identified as virulence factors of ExPEC, their contributions to bloodstream infection are still unclear. In this study, Vat(PU-1) and Tsh(PU-1) were verified to interact with the hemoglobin (a well-known mucin-like glycoprotein in red blood cell), degrade the mucins of host respiratory tract, and cleave the CD43 (a major cell surface component sharing similar O-glycosylated modifications with other glycoprotein expressed on leukocytes), suggesting that these two SPATEs have the common activity to cleave a broad array of mucin-like O-glycoproteins. These cleavages significantly impaired the chemotaxis and transmigration of leukocytes, and then inhibited the activation of diverse immune responses coordinately, especially downregulated the leukocytic and inflammatory activation during bloodstream infection, thus might mediate the evasion of ExPEC from immune clearance of blood leukocytes. Taken together, these two SPATEs play critical roles to cause a heavy bacterial load within bloodstream via immunomodulation of leukocytes, which provides a more comprehensive understanding how ExPEC colonize within host bloodstream and cause severe sepsis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台