Molecular characterization and ontogenetic expression profiles of LPXRFa and its receptor in Japanese flounder (Paralichthys olivaceus)
文献类型： 外文期刊
作者： Chen, Jun 1 ; Li, Yuru 1 ; Zhang, Wenwen 1 ; Zhao, Limiao 1 ; Huang, Xueying 1 ; Fang, Yan 1 ; Wang, Bin 2 ;
作者机构： 1.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
3.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China
关键词： LPXRFa; LPXRFa-R; Ontogenesis; Japanese flounder
期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.7； 五年影响因子：2.6 ）
ISSN： 0016-6480
年卷期： 2024 年 345 卷
页码：
收录情况： SCI
摘要： Investigations concerning the LPXRFa system are rarely conducted in flatfish species. Here, we first identified and characterized lpxrfa and its cognate receptor lpxrfa-r genes in the Japanese flounder (Paralichthys olivaceus). The coding DNA sequence of lpxrfa was 579 bp in length, wich encoded a 192-aa preprohormone that can produce three mature LPXRFa peptides. The open reading frame (ORF) of lpxrfa-r was 1446 bp in size, and encoded a 481-aa LPXRFa-R protein that encompassed seven hydrophobic transmembrane domains. Subse-quently, tissue distribution expression profiles of lpxrfa and lpxrfa-r transcripts were assayed by quantitative real-time PCR. The results indicated that expressions of lpxrfa transcripts were detected at the highest levels in the brain of both females and males, however, lpxrfa-r transcripts were remarkablely expressed in the brain tissue of female fish and in the testis tissue of male fish. Furthermore, transcript levels of lpxrfa and lpxrfa-r genes were investigated during early ontogenetic development, with the maximum expression levels at 30 days post -hatching. Overall, these data contribute to providing preliminary proof for the existence and structure of the LPXRFa system in Japanese flounder, and the study is just the foundation for researching physiological function of LPXRFa system in this species.
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