Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis
文献类型： 外文期刊
作者： Chen, Xiaolan 1 ; Wang, Zhijun 1 ; Chen, Yangfeng 1 ; Akinci, Ibrahim 6 ; Luo, Wei 8 ; Xu, Yibin 1 ; Jebessa, Endashaw 1 ; Blake, Damer 11 ; Sparks, Nick 12 ; Hanotte, Olivier 6 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Lingnan Guangdong Lab Modern Agr, Guangzhou, Guangdong, Peoples R China
2.South China Agr Univ, Coll Anim Sci, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Guangdong, Peoples R China
3.Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou, Guangdong, Peoples R China
4.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China
5.Chongqing Univ, Sch Life Sci, Chongqing, Peoples R China
6.Univ Nottingham, Sch Life Sci, Nottingham, England
7.Poultry Res Inst, Dept Anim Breeding & Genet, Ankara, Turkey
8.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Inst Anim Sci, Guangzhou, Peoples R China
9.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou, Peoples R China
10.Int Livestock Res Inst ILRI, LiveGene CTLGH, Addis Ababa, Ethiopia
11.Royal Vet Coll, Pathobiol & Populat Sci, N Mymms, England
12.Scotlands Rural Coll, Roslin Inst Bldg, Edinburgh, Scotland
关键词： avian coccidiosis; sasso chicken; circRNAs; circMGAT5; MMD
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：8.786； 五年影响因子：8.876 ）
ISSN： 1664-3224
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Avian coccidiosis is a common enzootic disease caused by infection of Eimeria species parasites. It causes huge economic losses in the global poultry industry. Current control using anticoccidial drugs or vaccination is limited due to drug resistance and the relatively high cost of vaccines. Improving host genetic resistance to Eimeria species is considered an effective strategy for improved control of coccidiosis. Circular RNAs (circRNAs) have been found to function as biomarkers or diagnoses of various kinds of diseases. The molecular biological functions of circRNAs, miRNAs, and mRNAs related to Sasso chicken have not yet been described during Eimeria species challenge. In this study, RNA-seq was used to profile the expression pattern of circRNAs, miRNAs, and mRNAs in spleens from Eimeria tenella-infected and non-infected commercial dual-purpose Sasso T445 breed chickens. Results showed a total of 40 differentially expressed circRNAs (DEcircRNAs), 31 differentially expressed miRNAs (DEmiRNAs), and 820 differentially expressed genes (DEmRNAs) between infected and non-infected chickens. Regulatory networks were constructed between differentially expressed circRNAs, miRNAs, and mRNAs to offer insights into the interaction mechanisms between chickens and Eimeria spp. Functional validation of a significantly differentially expressed circRNA, circMGAT5, revealed that circMGAT5 could sponge miR-132c-5p to promote the expression of the miR-132c-5p target gene monocyte to macrophage differentiation-associated (MMD) during the infection of E. tenella sporozoites or LPS stimulation. Pathologically, knockdown of circMGAT5 significantly upregulated the expression of macrophage surface markers and the macrophage activation marker, F4/80 and MHC-II, which indicated that circMGAT5 might inhibit the activation of macrophage. miR-132c-5p markedly facilitated the expression of F4/80 and MHC-II while circMGAT5 could attenuate the increase of F4/80 and MHC-II induced by miR-132c-5p, indicating that circMGAT5 exhibited function through the circMGAT5-miR-132c-5p-MMD axis. Together, our results indicate that circRNAs exhibit their resistance or susceptive roles during E. tenella infection. Among these, circMGAT5 may inhibit the activation of macrophages through the circMGAT5-miR-132c-5p-MMD axis to participate in the immune response induced by Eimeria infection.
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