Outer membrane vesicles secreted from Actinobacillus pleuropneumoniae isolate disseminating the floR resistance gene to Enterobacteriaceae
文献类型： 外文期刊
作者： Xu, Minsheng 1 ; Ke, Haiyi 5 ; Zang, Yingan 6 ; Gou, Hongchao 1 ; Yang, Dongxia 1 ; Shi, Keda 1 ; Zhang, Kunli 1 ; Li, Yan 1 ; Jiang, Zhiyong 1 ; Chu, Pinpin 1 ; Zhai, Shaolun 1 ; Li, Chunling 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China
2.Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Peoples R China
3.Guangdong Open Lab Vet Publ Hlth, Guangzhou, Peoples R China
4.Vet Drugs & Diagnost Tech Guangdong Prov, Sci Observat & Expt Stn, Guangzhou, Peoples R China
5.Guangdong Gaozhou Agr Sch, Maoming, Peoples R China
6.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou, Peoples R China
关键词： Actinobacillus pleuropneumoniae; whole genome shotgun; horizontal gene transfer; resistance plasmid; outer membrane vesicles (OMVs)
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）
ISSN：
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Actinobacillus pleuropneumoniae, a significant respiratory pig pathogen, is causing substantial losses in the global swine industry. The resistance spectrum of A. pleuropneumoniae is expanding, and multidrug resistance is a severe issue. Horizontal gene transfer (HGT) plays a crucial role in the development of the bacterial genome by facilitating the dissemination of resistance determinants. However, the horizontal transfer of resistance genes via A. pleuropneumoniae-derived outer membrane vesicles (OMVs) has not been previously reported. In this study, we used Illumina NovaSeq and PacBio SequeI sequencing platforms to determine the whole genome sequence of A. pleuropneumoniae GD2107, a multidrug-resistant (MDR) isolate from China. We detected a plasmid in the isolate named pGD2107-1; the plasmid was 5,027 bp in size with 7 putative open reading frames (ORF) and included the floR resistance genes. The carriage of resistance genes in A. pleuropneumoniae OMVs was identified using a polymerase chain reaction (PCR) assay, and then we thoroughly evaluated the influence of OMVs on the horizontal transfer of drug-resistant plasmids. The transfer of the plasmid to recipient bacteria via OMVs was confirmed by PCR. In growth competition experiments, all recipients carrying the pGD2107-1 plasmid exhibited a fitness cost compared to the corresponding original recipients. This study revealed that OMVs could mediate interspecific horizontal transfer of the resistance plasmid pGD2107-1 into Escherichia coli recipient strains and significantly enhance the resistance of the transformants. In summary, A. pleuropneumoniae-OMVs play the pivotal role of vectors for dissemination of the floR gene spread and may contribute to more antimicrobial resistance gene transfer in other Enterobacteriaceae.
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