Structural elucidation and immuno-stimulating activity of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba
文献类型: 外文期刊
作者: Du, XiuJu 1 ; Zhang, JingSong 1 ; Yang, Yan 1 ; Ye, LiBin 2 ; Tang, QingJiu 1 ; Jia, Wei 1 ; Liu, YanFang 1 ; Zhou, Shuai 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China
3.Shanghai Ocean Univ, Coll Food Sci, Shanghai 200090, Peoples R China
4.Liaocheng Univ, Coll Agr Sci, Liaocheng 252059, Shandong, Peoples R China
关键词: Tremella aurantialba;Polysaccharide;Structural elucidation;NMR spectroscopy;lmmuno-stimulating activity
期刊名称:CARBOHYDRATE RESEARCH ( 影响因子:2.104; 五年影响因子:2.192 )
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收录情况: SCI
摘要: A novel acidic heteropolysaccharide (TAPA1) was purified from hot water extracts of Tremella aurantialba fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-500 High-Resolution Chromatography. The heteropolysaccharide had a molecular weight of ca. 1.35 x 106 Da, and a carbohydrate content estimated to be -98.7% by the phenol-sulfuric acid method. It was composed mainly of o-mannose, o-xylose, and D_glucuronic acid in the ratio of ca. 5:4:1, along with trace amounts of D-galacturonic acid and o-glucose. Monosaccharide compositional analysis and GC-MS of methylated derivatives, combined with 1H and 13C NMR spectroscopies (including COSY, TOCSY, NOESY, HSQC, and HMBC spectra), revealed TAPA1 to consist of an α-(1_ú3)-linked mannopyranosyl backbone, partially substituted at position 4 with xylose side chains, and at position 2 with side chains composed of either xylose, mannose, and glucuronic acid or of xylose and mannose. Bioactivity testing showed that TAPA1 stimulated the proliferation of mouse spleen lymphocytes in vitro.
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