文献类型： 外文期刊

作者： Ma, Fuying ¹ ; Zhang, Fan ³ ; Zhu, Yu ¹ ; Lan, Dengyong ¹ ; Yan, Peiwen ¹ ; Wang, Ying ¹ ; Hu, Zejun ⁵ ; Zhang, Xinwei ¹ ; Hu, Jian ¹ ; Niu, Fuan ¹ ; Liu, Mingyu ¹ ; He, Shicong ¹ ; Cui, Jinhao ¹ ; Yuan, Xinyu ¹ ; Yan, Ying ⁵ ; Wu, Shujun ⁵ ; Cao, Liming ⁵ ; Bian, Hongwu ⁶ ; Yang, Jinshui ¹ ; Li, Zhikang ³ ; Luo, Xiaojin ¹ ;

作者机构： 1.Fudan Univ, State Key Lab Genet Engn, Shanghai 200438, Peoples R China

2.Fudan Univ, MOE Engn Res Ctr Gene Technol, Sch Life Sci, Shanghai 200438, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

4.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China

5.Shanghai Acad Agr Sci, Inst Crop Breeding & Cultivat, Shanghai 201403, Peoples R China

6.Zhejiang Univ, Inst Genet & Regenerat Biol, Coll Life Sci, Key Lab Cell & Gene Engn Zhejiang Prov, Hangzhou 310058, Peoples R China

7.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Minist Agr,Guangdong Lab Lingnan Modern Agr,Genome, Shenzhen 518100, Peoples R China

关键词： allele evolution; auxin signaling pathways; OsARFs; OsIAA10; yield traits

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Auxin is an important phytohormone in plants, and auxin signaling pathways in rice play key roles in regulating its growth, development, and productivity. To investigate how rice grain yield traits are regulated by auxin signaling pathways and to facilitate their application in rice improvement, we validated the functional relationships among regulatory genes such as OsIAA10, OsSK41, and OsARF21 that are involved in one of the auxin (OsIAA10) signaling pathways. We assessed the phenotypic effects of these genes on several grain yield traits across two environments using knockout and/or overexpression transgenic lines. Based on the results, we constructed a model that showed how grain yield traits were regulated by OsIAA10 and OsTIR1, OsAFB2, and OsSK41 and OsmiR393 in the OsSK41-OsIAA10-OsARF module and by OsARF21 in the transcriptional regulation of downstream auxin response genes in the OsSK41-OsIAA10-OsARF module. The population genomic analyses revealed rich genetic diversity and the presence of major functional alleles at most of these loci in rice populations. The strong differentiation of many major alleles between Xian/indica and Geng/japonica subspecies and/or among modern varieties and landraces suggested that they contributed to improved productivity during evolution and breeding. We identified several important aspects associated with the genetic and molecular bases of rice grain and yield traits that were regulated by auxin signaling pathways. We also suggested rice auxin response factor (OsARF) activators as candidate target genes for improving specific target traits by overexpression and/or editing subspecies-specific alleles and by searching and pyramiding the 'best' gene allelic combinations at multiple regulatory genes in auxin signaling pathways in rice breeding programs.