Characterization of small interfering RNAs derived from pepper mild mottle virus in infected pepper plants by high-throughput sequencing
文献类型： 外文期刊
作者： Jiao, Yubing 1 ; Zhao, Xiuxiang 1 ; Hao, Kaiqiang 1 ; Gao, Xinran 1 ; Xing, Dan 3 ; Wang, Zhiping 1 ; An, Mengnan 1 ; Xia, Zihao 1 ; Wu, Yuanhua 1 ;
作者机构： 1.Shenyang Agr Univ, Coll Plant Protect, Liaoning Key Lab Plant Pathol, Shenyang 110866, Liaoning, Peoples R China
2.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Pest Monitoring Controlling & Int, Qingdao 266101, Shandong, Peoples R China
3.Guizhou Acad Agr Sci, Inst Pepper, Guiyang 550006, Guizhou, Peoples R China
关键词： Pepper mild mottle virus; Virus-derived small interfering RNA; High-throughput sequencing; RNA silencing
期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）
ISSN： 0168-1702
年卷期： 2022 年 307 卷
页码：
收录情况： SCI
摘要： Pepper mild mottle virus (PMMoV) infects pepper plants and induces severe yield losses in China. However, the molecular interaction between PMMoV and pepper plants is largely unknown. RNA silencing is a eukaryotically conserved mechanism against viruses mediated by virus-derived small interfering RNAs (vsiRNAs) in plants. In this study, the profiles of vsiRNAs from PMMoV in infected pepper plants were obtained by high-throughput sequencing. The results showed that vsiRNAs were predominantly 21 and 22 nucleotides (nts) in length, and had a U bias at the 5'-terminal. The single-nucleotide resolution maps revealed that vsiRNAs were heterogeneously distributed throughout PMMoV genomic RNAs and hotspots of sense and antisense strands were mainly located in the RdRp and CP coding regions. The host transcripts targeted by vsiRNAs were predicted and they are mainly involved in physiological pathways related to stress response, cell regulation, and metabolism process. In addition, PMMoV infection induced significant up-regulation of CaAGO1a/1b/2, CaDCL2 and CaRDR1 gene transcripts in pepper plants, which are important components involved in antiviral RNA silencing pathway. Taken together, our results suggest the possible roles of vsiRNAs in PMMoV-pepper interactions.
- 相关文献
作者其他论文 更多>>
-
Effect of Phosphate Amendment on Cadmium Accumulation in Pepper (Capsicum annuum L.) Grown in Geogenic Cd-Rich Soil from the Karst Region
作者：Li, Yuyang;Zhou, Wangwang;Zhang, Yongxiang;Liu, Yizhang;Xing, Dan;Wen, Rong;Wen, Rong;Zhou, Wangwang;Zhang, Yongxiang
关键词：soil pollution; heavy metal; Cd accumulation; phosphorus; food crops
-
Physiological and biochemical responses of pepper (Capsicum annuum) to cadmium stress: The mitigating effects of exogenous abscisic acid
作者：Tu, Dehui;Tian, Xiao;Liang, Chuangjing;Zhou, Peng;Wang, Yongping;Xing, Dan;Liu, Yizhang
关键词：Cell wall; Abscisic acid; Oxidative stress; Cd stress; Subcellular distribution
-
Dietary Sodium Butyrate Supplementation Enhances Silkworm Silk Yield by Simultaneously Promoting Larval Growth and Silk Gland Development
作者：Ren, Xiaoxiao;Ye, Zhanfeng;Qing, Zhuo;Yang, Wanjun;Luo, Chaobin;Xing, Dan;He, Xingjiang
关键词：silkworm; sodium butyrate; silk yield; body growth; silk gland development
-
Arbuscular mycorrhizal fungi decrease soil nitrate by accelerating mulberry uptake and dissimilatory nitrate reduction to ammonium
作者：Xing, Dan;Zhang, Huirong;Zhang, Fang;Shi, Yanjin;Peng, Shiqing;Luo, Chaobin;Zhang, Huirong;Cheng, Hongguang;Twagirayezu, Gratien
关键词：Soil Nitrate; Arbuscular Mycorrhizal Fungi; Plants; Nitrogen Cycle; Microorganisms
-
Effects of fungicide application on susceptible pepper cultivar under southern blight stress
作者：Wen, Rong;Xing, Dan;Wen, Rong;Liang, Chuanjing;Wang, Yongping;Tu, Dehui;Luo, Zhenyi;Xing, Dan;Song, Dandan;Wu, Zhibing;Twagirayezu, Gratien;Zhang, Shicai;Wu, Kangyun
关键词：Sclerotium rolfsii sacc; Pepper southern blight; Susceptible cultivar; Fungicide; Root system
-
Increased nitrogen accumulation in mulberry trees due to the secretion of glomalin-related soil protein induced by arbuscular mycorrhizal fungi
作者：Zhang, Huirong;Zhang, Fang;Peng, Shiqing;Shi, Yanjin;Luo, Chaobin;Xing, Dan;Zhang, Huirong;Wang, Zhenhong;Cheng, Hongguang;Tian, Xueping
关键词：Arbuscular mycorrhizal fungi; Nitrogen nutrition; Soil inorganic nitrogen; Soil dissolved organic nitrogen
-
Application of thifluzamide to stem rot in peppers: Infection and control mechanisms of sclerotium rolfsii
作者：Song, Dandan;Chen, Kuai;Wen, Fanglin;Wu, Zhibing;Wen, Rong;Xing, Dan;Wen, Rong
关键词：Infection mechanism; Pepper; Sclerotium rolfsii; Stem rot disease; Thifluzamide
