您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of small interfering RNAs derived from pepper mild mottle virus in infected pepper plants by high-throughput sequencing

文献类型： 外文期刊

作者： Jiao, Yubing 1 ; Zhao, Xiuxiang 1 ; Hao, Kaiqiang 1 ; Gao, Xinran 1 ; Xing, Dan 3 ; Wang, Zhiping 1 ; An, Mengnan 1 ; Xia, Zihao 1 ; Wu, Yuanhua 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Plant Protect, Liaoning Key Lab Plant Pathol, Shenyang 110866, Liaoning, Peoples R China

2.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Pest Monitoring Controlling & Int, Qingdao 266101, Shandong, Peoples R China

3.Guizhou Acad Agr Sci, Inst Pepper, Guiyang 550006, Guizhou, Peoples R China

关键词： Pepper mild mottle virus; Virus-derived small interfering RNA; High-throughput sequencing; RNA silencing

期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）

ISSN： 0168-1702

年卷期： 2022 年 307 卷

页码：

收录情况： SCI

摘要： Pepper mild mottle virus (PMMoV) infects pepper plants and induces severe yield losses in China. However, the molecular interaction between PMMoV and pepper plants is largely unknown. RNA silencing is a eukaryotically conserved mechanism against viruses mediated by virus-derived small interfering RNAs (vsiRNAs) in plants. In this study, the profiles of vsiRNAs from PMMoV in infected pepper plants were obtained by high-throughput sequencing. The results showed that vsiRNAs were predominantly 21 and 22 nucleotides (nts) in length, and had a U bias at the 5'-terminal. The single-nucleotide resolution maps revealed that vsiRNAs were heterogeneously distributed throughout PMMoV genomic RNAs and hotspots of sense and antisense strands were mainly located in the RdRp and CP coding regions. The host transcripts targeted by vsiRNAs were predicted and they are mainly involved in physiological pathways related to stress response, cell regulation, and metabolism process. In addition, PMMoV infection induced significant up-regulation of CaAGO1a/1b/2, CaDCL2 and CaRDR1 gene transcripts in pepper plants, which are important components involved in antiviral RNA silencing pathway. Taken together, our results suggest the possible roles of vsiRNAs in PMMoV-pepper interactions.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台