Temporal variation in DNA methylation during gonadal development in a reptile with temperature-dependent sex determination(dagger)
文献类型： 外文期刊
作者： Liu, Fang 1 ; Xu, Haoyang 1 ; Ni, Wei 1 ; Wang, Yakun 1 ; Hong, Xiaoyou 1 ; Li, Wei 1 ; Yu, Lingyun 1 ; Chen, Chen 1 ; Wei, Chengqing 1 ; Liu, Xiaoli 1 ; Zhu, Xinping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr & Rural Affairs, Guangzhou 51038, Peoples R China
2.Shanghai Ocean Univ, Coll Life Sci & Fisheries, Shanghai 201306, Peoples R China
关键词： DNA methylation; temperature-dependent sex determination; sexual development; sex-related genes; Mauremys mutica
期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：4.161； 五年影响因子：4.522 ）
ISSN： 0006-3363
年卷期：
页码：
收录情况： SCI
摘要： DNA methylation plays a significant role in transducing external environmental signals to a cellular response in reptiles; however, whether the methylation patterns are conserved across species remains unclear. Here, we examined the genome-wide DNA methylation differentiation between male and female hatchling gonads of the temperature-dependent sex determination (TSD) Mauremys mutica (M. mutica) using methylation-dependent restriction-site associated DNA sequencing (MethylRAD-seq) to test differentially methylated genes underlying sexual development. Several categories, including heat-shock genes (HSP90A, HSP30C), histone- (KDM8) and ubiquitin-related genes (TRIM39), kinases (WNK3), and gonad differentiation or gonadal-development-related genes (HSD17B8, HSD17B12), were identified as candidates for future study. Additionally, we identified several regulatory pathways potentially mediating TSD thermosensitivity such as the GnRH signaling pathway and calcium signaling pathway. These findings provide evidence that sexually dimorphic DNA methylation may be associated with sex determination or sex differentiation in TSD M. mutica. Affected by temperature, several genes (heat-shock genes, histone- and ubiquitin-related genes, kinase-related genes), and pathways (GnRH signaling pathway and calcium signaling pathway) of TSD (M. mutica) may change the methylation level of the regulatory regions, which revealed that sexually dimorphic DNA methylation could act as a key mediator regulating thermosensitive signals into a molecular trigger to determine sexual fate.
- 相关文献
作者其他论文 更多>>
-
Assessing and Screening of Female Fertility in Artificially Bred Asian Yellow Pond Turtles (Mauremys mutica) Based on Parentage Assignment
作者：Zhang, Xincheng;Zhao, Jian;Zhou, Chenyao;Li, Wei;Liu, Yihui;Wei, Chengqing;Wang, Yakun;Hong, Xiaoyou;Zhu, Xinping;Liu, Xiaoli;Zhou, Chenyao
关键词：female fecundity; Mauremys mutica; microsatellite; breeder contributions; genetic variability
-
Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection
作者：Ji, Liqin;Chen, Chen;Zhu, Junxian;Hong, Xiaoyou;Liu, Xiaoli;Wei, Chengqing;Zhu, Xinping;Li, Wei
关键词：Chinese soft-shelled turtle; plasma parameters; transcriptome; metabolome; liver; Aeromonas hydrophila
-
Assessment of benthic ecological status and heavy metal contamination in an estuarine intertidal mudflat in the Northern Bohai Sea
作者：Li, Ang;Li, Jiaqi;Liu, Lulei;Xue, Suyan;Zhang, Meng;Tang, Yuze;Mao, Yuze;Li, Ang;Li, Jiaqi;Zhu, Ling;Liu, Lulei;Xue, Suyan;Zhang, Meng;Tang, Yuze;Mao, Yuze;Liu, Fang
关键词：Geligang; Macrobenthos; Benthic ecological quality; M-AMBI; Heavy metals
-
Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica)
作者：Liu, Xiaoli;Xu, Haoyang;Peng, Mingwei;Zhou, Chenyao;Wei, Chengqing;Hong, Xiaoyou;Li, Wei;Chen, Chen;Ji, Liqin;Zhu, Xinping;Liu, Xiaoli;Xu, Haoyang;Li, Wei;Zhu, Xinping;Peng, Mingwei;Zhou, Chenyao;Zhu, Xinping
关键词：Turtle; Gonadal transcriptome; RNA-seq; Sexual dimorphism; Temperature-dependent sex determination
-
Development and characterization of 68 SNPs markers in the critically endangered species, Palea steindachneri
作者：Zhu, Junxian;Liu, Pan;Ji, Liqin;Hong, Xiaoyou;Liu, Xiaoli;Chen, Haigang;Wei, Chengqing;Zhu, Xinping;Chen, Chen;Li, Wei;Zhu, Junxian;Zhu, Xinping;Li, Wei
关键词：Palea steindachneri; Critically endangered species; Genetic resources conservation; SNP marker
-
Effects of Photoperiod on Survival, Growth, Physiological, and Biochemical Indices of Redclaw Crayfish (Cherax quadricarinatus) Juveniles
作者：Nie, Xiangxing;Huang, Cuixue;Chu, Zhangjie;Nie, Xiangxing;Huang, Cuixue;Wei, Jie;Wang, Yakun;Hong, Kunhao;Zhu, Xinping;Yu, Lingyun;Mu, Xidong;Liu, Chao
关键词：aquaculture; circulating water culture; environmental factor; body color; gene expression
-
Identification of Sex-Specific Markers and Candidate Genes Using WGS Sequencing Reveals a ZW-Type Sex-Determination System in the Chinese Soft-Shell Turtle (Pelodiscus sinensis)
作者：Zhu, Junxian;Zhu, Xinping;Li, Wei;Zhu, Junxian;Wang, Yongchang;Chen, Chen;Ji, Liqin;Hong, Xiaoyou;Liu, Xiaoli;Chen, Haigang;Wei, Chengqing;Zhu, Xinping;Li, Wei
关键词：Pelodiscus sinensis; whole-genome sequencing; sex-specific sequence screening; sex-specific markers; sex-determining candidate genes
