文献类型： 外文期刊

作者： Wang, Xiao-Bo ¹ ; Lu, Hong-Wei ¹ ; Liu, Qing-You ³ ; Li, A-Lun ¹ ; Zhou, Hong-Ling ¹ ; Zhang, Yong ⁴ ; Zhu, Tian-Qi ⁶ ; Ruan, Jue ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab,M, Shenzhen 518120, Guangdong, Peoples R China

2.Henan Acad Agr Sci, Inst Crop Mol Breeding, Shennong Lab, Zhengzhou 450002, Peoples R China

3.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan 528225, Peoples R China

4.Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China

5.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects &, Beijing 100101, Peoples R China

6.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Beijing 100190, Peoples R China

7.Chinese Acad Sci, Acad Math & Syst Sci, Key Lab Random Complex Struct & Data Sci, Beijing 100190, Peoples R China

关键词： sex-limited chromosome; assembly method; long-read sorting

期刊名称：GIGASCIENCE （ 影响因子：11.8； 五年影响因子：9.4 ）

ISSN： 2047-217X

年卷期： 2024 年 13 卷

页码：

收录情况： SCI

摘要： Background Most currently available reference genomes lack the sequence map of sex-limited (such as Y and W) chromosomes, which results in incomplete assemblies that hinder further research on sex chromosomes. Recent advancements in long-read sequencing and population sequencing have provided the opportunity to assemble sex-limited chromosomes without the traditional complicated experimental efforts.Findings We introduce the first computational method, Sorting long Reads of Y or other sex-limited chromosome (SRY), which achieves improved assembly results compared to flow sorting. Specifically, SRY outperforms in the heterochromatic region and demonstrates comparable performance in other regions. Furthermore, SRY enhances the capabilities of the hybrid assembly software, resulting in improved continuity and accuracy.Conclusions Our method enables true complete genome assembly and facilitates downstream research of sex-limited chromosomes.