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Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn

文献类型: 外文期刊

作者: Sun, Yaning 1 ; Hu, Xiaofei 1 ; Zhang, Yong 2 ; Yang, Jifei 1 ; Wang, Fangyu 1 ; Wang, Yao 1 ; Deng, Ruiguang 1 ; Zhang, 1 ;

作者机构: 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

关键词: zearalenone;monoclonal antibody;test strip;colloidal gold;immunoassay

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether was prepared as immunogen. The mAb showed low cross-reactivity with five ZEN analogues. Using an antibody preparation with a titer of >= 1:5.12 x 10(5), the cross-reactivity (CR) of the anti-ZEN monoclonal antibody with four of the analogues was <496, except for zearalanone, which was 53.121%. The recovery rates of ZEN in spiked corn samples were in the range of 91.30-97.07% with coefficients of variation <5.32%. An immunochromatographic strip was developed using the specific anti-ZEN monoclonal antibody and applied to the screening of corn samples for ZEN residues. The test could be accomplished within 5-10 min. The sensitivity of the test strip in corn sample extract was confirmed to be 20 mu g/kg by unaided visual assessment, and the IC50 was calculated as 3.4 ng/mL using a test strip reader. The test strip, analyzed by unaided visual assessment and strip reader, showed very good agreement with competitive indirect ELISA and high-performance liquid chromatography (HPLC) analysis for naturally contaminated corn samples.

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