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Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal

文献类型: 外文期刊

作者: Sun, Yaning 1 ; Xing, Guangxu 1 ; Yang, Jifei 1 ; Wang, Fangyu 1 ; Deng, Ruiguang 1 ; Zhang, Gaiping 1 ; Hu, Xiaofei 1 ;

作者机构: 1.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China

关键词: ochratoxin A;zearalenone;colloidal gold;immunoassay

期刊名称:JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE ( 影响因子:3.638; 五年影响因子:3.802 )

ISSN:

年卷期:

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收录情况: SCI

摘要: BACKGROUNDOchratoxin A (OTA) and zearalenone (ZEN) are natural products of filamentous fungi that are harmful to humans and animals exposed to them even in extremely low concentration. The immunochromatographic test strip has become a popular diagnostic tool for detecting analytes. Its major advantages are that results can be obtained within 5-10 min, all needed reagents are included in the strip and it can be used to detect OTA and ZEN contamination in spots. In this study a colloidal gold-based immunochromatographic test strip of competitive format was developed for the rapid simultaneous qualitative and quantitative detection of OTA and ZEN in corn and other cereals.

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