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Effect of non-permeable cryoprotectant sucrose on the development of spotted knifejaw (Oplegnathus punctatus) embryos

文献类型： 外文期刊

作者： Li, Linlin 1 ; Tian, Yongsheng 1 ; Li, Zhentong 1 ; Duan, Pengfei 1 ; Wang, Xinyi 1 ; Chen, Shuai 1 ; Wang, Linna 1 ; Liu, Yang 1 ; Wang, Qingbin 4 ; Li, Wensheng 4 ; Zhao, Xia 4 ; Ma, Wenhui 4 ; Zhai, Jieming 4 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China

4.Laizhou Mingbo Aquat Co Ltd, Yantai 2614000, Peoples R China

5.Chinese Acad Fishery, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao, Peoples R China

关键词： Sucrose; Toxicity; Non-permeable cryoprotectant; Oplegnathus punctatus; Rehydration; Embryo; Malformation

期刊名称：CRYOBIOLOGY （ 影响因子：2.7； 五年影响因子：2.7 ）

ISSN： 0011-2240

年卷期： 2023 年 112 卷

页码：

收录情况： SCI

摘要： In this study, the toxicity of sucrose to Oplegnathus punctatus embryos was evaluated. Embryos at the 4-6 somite, tail-bud, heart formation, and heart-beating stages were exposed to 0, 0.5, 1,1.5, 2, 2.5, or 3 M sucrose for 1 h. Survival rates of embryos at the tail-bud, heart formation, and heart-beating stages after rehydration for 1 h were not affected by treatment with 2 M sucrose (the maximum concentration). Embryos at the tail-bud, heart formation, and heart-beating stages were exposed to 2 M sucrose for 0, 30, 60, 90, 120, 150, or 180 min. Long-term developmental indicators, including rates of survival, hatching, swimming, and malformation, were evaluated for 4 days after rehydration. Based on the survival rates 10 min after rehydration, the longest tolerance time for embryos at the three stages was 120 min. Based on long-term developmental indicators, the longest tolerance times were 60 min at the tail-bud, 60 min at the heart formation stage and 30 min at the heart beating stage. The malformation rates increased as the treatment time increased. The malformation rates were 100% when embryos were exposed to sucrose for & GE;120 min. Malformation was divided into larval and embryonic abnormality. As the exposure time increased for tail-bud stage embryos, the rate of larval malformation increased. Treatment at heart formation and heart-beating stages resulted in higher rates of failure to hatch at exposure time. Based on these results, toxicity tests of non-permeable cryoprotectant in embryos requires the observation of development for at least 2 days after rehydration. Based on long-term observation, it was concluded that dehydration before freezing was not the direct cause of larvae deformity that hatched from frozen-thawing embryo. These results provide a reference for the singly use of representative non-permeable cryoprotectant sucrose.

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