iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds
文献类型： 外文期刊
作者： Luo, Yongjian 1 ; Zhang, Yixin 2 ; Jiang, Yu 1 ; Dai, Zhangyan 2 ; Li, Qing 2 ; Mou, Jiaolin 1 ; Xu, Li 1 ; Deng, Shiming 1 ; Li, Jitao 1 ; Wang, Ru 1 ; Liu, Jun 2 ; Deng, Zhijun 1 ;
作者机构： 1.Hubei Minzu Univ, Hubei Key Lab Biol Resources Protect & Utilizat, Enshi 445000, Peoples R China
2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Peoples R China
3.Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China
4.Hubei Minzu Univ, Res Ctr Germplasm Engn Characterist Plant Resource, Enshi 445000, Peoples R China
5.Hubei Minzu Univ, Sch Forestry & Hort, Plant Germplasm Resources Lab, Enshi 445000, Peoples R China
关键词： DEP; germination; LEA; orthodox seeds; reactive oxygen species (ROS); HSP
期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 12 卷 9 期
页码：
收录情况： SCI
摘要： Sophora tonkinensi is a shrub of the genus Sophora in the family Fabaceae with anti-inflammatory and pain-relieving effects. While the cultivation, chemical makeup, and medicinal properties of S. tonkinensis have been reported, the physiological mechanisms governing its dehydration and cryopreservation tolerance of seeds remain unclear. In this study, we investigated the morphological, physiological, biochemical, and protein expression characteristics of S. tonkinensis seeds subjected to dehydration and cryopreservation techniques via the observation of cell microstructure, determination of antioxidant enzyme activity, and iTRAQ-based proteomic analysis, respectively. The results of the study demonstrated that the seeds possessed a certain level of tolerance to dehydration. The highest germination percentage of 83.0% was observed after 2 h of dehydration (10.1% water content), which was identified as the optimal time point for cryopreservation. However, the germination percentage was reduced to only 30.5% when the water content reached 5.4%, indicating that S. tonkinensis seeds exhibit intermediate storage behavior. Further investigation revealed that during seed dehydration and cryopreservation treatment, liposomes were gradually and highly fused, whereas the activities of ROS scavenging and stress defense were significantly enhanced. During dehydration, the seed tissues formed a protective mechanism of stress resistance based on protein processing in the endoplasmic reticulum and antioxidant system, which was related to the dehydration tolerance. Moreover, only three differentially expressed LEA proteins were identified, and it is speculated that the strengthening of intracellular metabolism and the absence of specific LEA and dehydrins could be crucial factors for the reduced germination percentage after excessive dehydration and cryopreservation.
- 相关文献
作者其他论文 更多>>
-
Experimental Warming Increased Cooked Rice Stickiness and Rice Thermal Stability in Three Major Chinese Rice Cropping Systems
作者：Yang, Huifang;Zhang, Bin;Yang, Taotao;Yang, Huifang;Chen, Liming;Xiong, Ruoyu;Zeng, Yanhua;Xiong, Ruoyu;Zeng, Yanhua;Jiang, Yu;Zhang, Jun
关键词：climate warming; cropping systems; rice quality; starch structure
-
Calcium chloride treatment delays the quality deterioration of king oyster mushroom ( Pleurotus eryngii) ) through positive regulation of antioxidants by PeCaMK1
作者：Lian, Lingdan;Zhou, Youhua;Du, Minru;Lin, Yimei;Chang, Hao;Zhang, Shengxian;Wang, Jie;Liu, Jun
关键词：PeCaMK1; Postharvest quality; Antioxidant enzymes; Pleurotus eryngii; Ca2+ signaling
-
Occurrence of Leaf Spot on Elaeocarpus decipiens Caused by Alternaria alternata, a New Disease in China
作者：Liu, Jun;Zhou, Shengyang;Zhang, Yehui;Lian, Lingdan
关键词：Alternaria alternata; Elaeocarpus decipiens; leaf spot
-
Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd
作者：Zeng, Lingen;Deng, Zhijun;Zeng, Lingen;Zhang, Hui;Liu, Hongbiao;Du, Hu;Zhong, Yujuan;Yao, Chunpeng;Zhang, Hui;Guo, Jinju;Zhang, Changyuan
关键词：bitter gourd; virus-induced gene silencing; TRSV; phytoene dehydrogenase
-
A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis
作者：Liu, Yujuan;Zhang, Zhi;Liu, Jinyuan;Wang, Changhu;Chen, Chen;Liu, Yujuan;Shu, Jie;Zhang, Zhi;Ding, Ning;Liu, Jinyuan;Wang, Changhu;Chen, Chen;Liu, Yujuan;Shu, Jie;Zhang, Zhi;Ding, Ning;Liu, Jinyuan;Wang, Changhu;Chen, Chen;Liu, Yujuan;Zhang, Zhi;Liu, Jinyuan;Wang, Changhu;Chen, Chen;Shu, Jie;Liu, Jun;Ding, Ning;Cui, Yuhai;Cui, Yuhai
关键词：
-
Advance in the Thermoinhibition of Lettuce (Lactuca sativa L.) Seed Germination
作者：Wei, Jinpeng;Zhang, Qi;Zhang, Yixin;Yang, Le;Chen, Bingxian;Wei, Jinpeng;Yang, Le;Zhou, Yuliang;Zeng, Zhaoqi
关键词：lettuce seeds; high-temperature germination; thermoinhibition; hormones; interactions; genetic expression
-
Experimental Warming Reduces the Grain Yield and Nitrogen Utilization Efficiency of Double-Cropping indica Rice in South China
作者：Yang, Taotao;Zou, Jixiang;Wu, Longmei;Bao, Xiaozhe;Zhang, Bin;Jiang, Yu;Zhang, Nan
关键词：climate warming; double-cropping indica rice; grain yield; nitrogen uptake; nitrogen utilization efficiency
