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The key technologies of Ganoderma lucidum liquid spawn preparation and scale expansion

文献类型： 外文期刊

作者： Guo, Jia 1 ; Liu, Yan-Fang 1 ; Tang, Chuan-Hong 1 ; Zhang, Jing-Song 1 ; Feng, Jie 1 ;

作者机构： 1.Shanghai Acad Agr Sci, China Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South,Ke, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

关键词： Fermentation scale; Ganoderma lucidum; Liquid spawn; Metabolic activity

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 0959-3993

年卷期： 2023 年 39 卷 6 期

页码：

收录情况： SCI

摘要： Ganoderma lucidum possesses a variety of valuable pharmacological activities, and it has long been used to prevent and treat various human diseases. Up to now, far too little attention has been paid to the liquid spawn of G. lucidum, and the development of the G. lucidum industry is constrained by them. This work aimed to study the key technologies and scale-up preparation of G. lucidum liquid spawn, to achieve large-scale preparation of liquid spawn and solve the problem of unstable quality of G. lucidum. The plate culture, primary shake flask culture, shake flask preparation, and fermentor preparation of G. lucidum liquid spawn were explored in the process of liquid fermentation. The results showed that plate broth volume significantly affected mycelial growth rate. Biomass in the primary shake flask culture is significantly influenced by the picking position of plate mycelium. An artificial neural network coupled with a genetic algorithm was used for carbon and nitrogen sources concentration optimization to increase biomass and substrate utilization. The optimized parameter combination is as follows: glucose, 14.5 g L-1; yeast extract powder, 8.5 g L-1. Under this condition, the biomass (9.82 g L-1) and biomass on reducing sugar (0.79 g g(-1)) increased by 18.03% and 27.41% compared to the control, respectively. The metabolic activity of liquid spawn prepared by different fermentation scales was diverse, and the liquid spawn prepared by the fermentor has better activity. Conceivably, the liquid spawn process can more conducive be applied to large-scale industrial production.

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