Novel insights into the inhibitory effect of pterostilbene targeting Aeromonas hydrophila virulence
文献类型： 外文期刊
作者： Dong, Jing 1 ; Zhou, Shun 1 ; Liu, Yongtao 1 ; Yang, Qiuhong 1 ; Yang, Yibin 1 ; Xu, Ning 1 ; Ai, Xiaohui 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
关键词： Pterostilbene; Aerolysin; Quorum sensing; Molecular dynamics; Aeromonas hydrophila
期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.1 ）
ISSN： 0044-8486
年卷期： 2024 年 590 卷
页码：
收录情况： SCI
摘要： The overuse of antibiotics in aquaculture brings a range of problems. Therefore, novel approaches alternating antibiotics are urgently needed. Strategies targeting bacterial virulence have become one of the routes developing drugs against bacterial infections. Here, we found that pterostilbene (PSE) could inhibit the activity of aerolysin (AerA) and disrupt quorum sensing (QS) at sub-inhibitory concentrations. Molecular docking and dynamics simulation assays were performed to predict the binding mode and binding sites of AerA-PSE complex. Then we confirmed that PSE bound to the sites of Val189 and Ile302 and prevented AerA forming heptamer with transmembrane pores. In addition, PSE could protect to A549 cells and the channel catfish (Ictalurus punctatus) against AerA mediated cell injury and mortality infected with A. hydrophila. The findings demonstrated that targeting bacterial virulence could be a promising measure identifying agents against A. hydrophila infections.
- 相关文献
作者其他论文 更多>>
-
Molecular mechanism of Yersinia ruckeri Flagellin C (FliC) induced intestinal inflammation in channel catfish ( Ictalurus punctatus)
作者：Yang, Yibin;He, Hao;Ai, Xiaohui;Zhu, Xia;Huang, Yucong;Yin, Fei;Xu, Jingen;Chen, Yuhua
关键词：Channel catfish; Yersinia ruckeri; Virulence; Intestinal inflammation; Molecular mechanism
-
Characteristics and functions of interleukin 21 in channel catfish (Ictalurus punctatus)
作者：He, Hao;He, Hao;Zhu, Xia;Dong, Jing;Yang, Qiuhong;Ai, Xiaohui;Yang, Yibin;Deng, Ping;Huang, Yucong;Wang, Hui
关键词：Channel catfish; IL-21; Immune response; Inflammatory factors; Gene expression
-
Population Pharmacokinetics of Enrofloxacin in Ctenopharyngodon idella Based on the Sparse Sampling Method and a Nonlinear Mixed Effect Model Following Intravenous and Oral Administration
作者：Xu, Ning;Zhang, Huan;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Xu, Ning;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Zhang, Huan;Ding, Yongzhen
关键词：bioavailability; enrofloxacin; grass carp; population pharmacokinetics
-
Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass
作者：Yang, Yibin;He, Hao;Yang, Qiuhong;Ai, Xiaohui;Zhu, Xia;Deng, Ping
关键词：Streptococcus iniae; Largemouth bass; Whole genome; Virulence; Drug resistance
-
Population Pharmacokinetics of Enrofloxacin in Micropterus salmoides Based on a Nonlinear Mixed Effect Model After Intravenous and Oral Administration
作者：Xu, Ning;Zhou, Shun;Dong, Jing;Li, Jiangtao;Ai, Xiaohui;Xu, Ning;Ding, Yongzhen
关键词：enrofloxacin; population pharmacokinetics; statistical approaches; largemouth bass; body weight
-
Preclinical Evaluation of Fenbendazole for Controlling Gyrodactylus kobayashii (Monogenea, Gyrodactylidae) in Goldfish: Dose Optimization and Safety Assessment
作者：Dong, Jing;Li, Jiangtao;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Ai, Xiaohui;Zhou, Shun;Dong, Jing
关键词：preclinical study; fenbendazole; monogenean; anthelmintic treatment; aquaculture
-
Novel Insights into the Therapeutic Effect of Amentoflavone Against Aeromonas hydrophila Infection by Blocking the Activity of Aerolysin
作者：Dong, Jing;Li, Shengping;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Yang, Yibin;Ai, Xiaohui;Li, Shengping;Cheng, Bo
关键词：
Aeromonas hydrophila ; aerolysin; amentoflavone; molecular dynamics; anti-infective
