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iTRAQ proteomics reveals the regulatory response to Xanthomonas campestris pv. vesicatoria in resistant vs. susceptible pepper genotypes

文献类型： 外文期刊

作者： Wang, Fei 1 ; Gao, Shenghua 1 ; Niran, Juntawong 2 ; Li, Ning 1 ; Yin, Yanxu 1 ; Yu, Chuying 1 ; Jiao, Chunhai 1 ; Yao, Minghua 1 ;

作者机构： 1.Hubei Acad Agr Sci, Cash Crops Res Inst, Hubei Key Lab Vegetable Germplasm Enhancement & Ge, Wuhan 430070, Hubei, Peoples R China

2.Kasetsart Univ, Bangkok 10900, Thailand

关键词： Pepper; Proteomics analysis; Bacterial spot; Xanthomonas campestris pv; vesicatoria

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：4.24； ）

ISSN： 2095-9885

年卷期： 2022 年 8 卷 6 期

页码：

收录情况： SCI

摘要： Bacterial spot (BS) is a severe bacterial disease induced by Xanthomonas campestris pv. vesicatoria (Xcv), a pathogen that causes serious damage to pepper growth and yield. It is therefore important to study the mechanisms of pepper resistance to Xcv and to breed and promote Xcv-resistant pepper varieties. However, studies of the responses to Xcv infection in peppers at the protein level are limited. Here, we examined Xcv-induced proteomic changes in leaves of the BS susceptible bell pepper ECW and the resistant bell pepper VI037601 using the isobaric tags for relative and absolute quantitation (iTRAQ)-based protein labeling technology. A total of 6,120 distinct proteins were identified, and there were 1,289 significantly differentially accumulated proteins (DAPs) in ECW and VI037601 leaves after Xcv inoculation. Among these, 339 (250 up-and 89 down-regulated) and 479 (300 up-and 179 down-regulated) DAPs were specifically identified in ECW and VI037601, respectively, with 459 (364 up-and 95 down-regulated) similarly expressed DAPs being shared by ECW and VI037601. Based on bioinformatics analysis, many defense-associated proteins were identified as up-regulated in ECW and VI037601, especially the proteins involved in plant-pathogen interaction, phenylpropanoid biosynthesis, protein processing in the endoplasmic reticulum, and MAPK signaling pathway -plant. Moreover, we evaluated transcript levels of six differentially expressed genes from the iTRAQ results by qRT-PCR. The analysis revealed transcriptional changes that were consistent with the changes at the protein level. This study will provide a valuable resource for understanding the molecular basis of pepper resistance to Xcv infection and for improving the disease resistance of pepper cultivars.

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