Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc x Guangdong small spotted pig
文献类型： 外文期刊
作者： Wu, Zhuosui 1 ; Wang, Zhonggang 2 ; Wang, Pan 1 ; Cheng, Leiyan 1 ; Li, Jianhao 1 ; Luo, Yanfeng 3 ; Yang, Linfang 3 ; Li, Linfeng 1 ; Zeng, Jianhua 3 ; Hu, Bin 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Guangdong, Peoples R China
2.Guangdong Guanghong Agr & Anim Husb Dev Co Ltd, Huizhou, Peoples R China
3.Guangdong Yihao Foodstuff Co Ltd, Zhanjiang, Peoples R China
关键词： fat deposition; lipidomics; proteomics; metabolic pathways; Duroc x Guangdong small spotted pig
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）
ISSN：
年卷期： 2024 年 11 卷
页码：
收录情况： SCI
摘要： Introduction This study aims to explore the important factors affecting the characteristics of different parts of pork.Methods Lipidomics and proteomics methods were used to analyze DAL (differential lipids) and DAPs (differential proteins) in five different parts (longissimus dorsi, belly meat, loin, forelegs and buttocks) of Duhua pig (Duroc x Guangdong small spotted pig), to identify potential pathways affecting meat quality, investigating fat deposition in pork and its lipid-protein interactions.Results The results show that TG (triglyceride) is the lipid subclass with the highest proportion in muscle, and the pathway with the most significantly enriched lipids is GP. DAP clustered on several GO terms closely related to lipid metabolism and lipogenesis (lipid binding, lipid metabolism, lipid transport, and lipid regulation). In KEGG analysis, there are two main DAP aggregation pathways related to lipid metabolism, namely Fatty acid degradation and oxidative phosphorylation. In PPI analysis, we screened out 31 core proteins, among which NDUFA6, NDUFA9 and ACO2 are the most critical.Discussion PC (phosphatidylcholine) is regulated by SNX5, THBS1, ANXA7, TPP1, CAVIN2, and VDAC2 in the phospholipid binding pathway. TG is regulated by AUH/HADH/ACADM/ACADL/HADHA in the lipid oxidation and lipid modification pathways. Potential biomarkers are rich in SFA, MUFA and PUFA respectively, the amounts of SFA, MUFA and PUFA in the lipid measurement results are consistent with the up- and down-regulation of potential biomarker lipids. This study clarified the differences in protein and lipid compositions in different parts of Duhua pigs and provided data support for revealing the interactions between pork lipids and proteins. These findings provide contributions to the study of intramuscular fat deposition in pork from a genetic and nutritional perspective.
- 相关文献
作者其他论文 更多>>
-
Accumulation and distribution of cadmium at organic-mineral micro-interfaces across soil aggregates
作者：Li, Qi;Li, Linfeng;Lin, Xiaoyang;Xiao, Anwen;Li, Yichun;Yin, Bohao;Xue, Weijie;Liu, Hengjie
关键词：Soil amendment; Cd distribution; Organic components; Fe oxides; Aggregates; NanoSIMS
-
Regulation of soil properties by amendments and their impact on Cd fractions and bacterial community structure: Exploring the mechanism of inhibition on Cd phytoavailability
作者：Li, Linfeng;Li, Qi;Xiao, Anwen;Li, Chonghua;Li, Yichun;Li, Linfeng;Li, Qi;Xiao, Anwen;Li, Chonghua;Li, Yichun;Li, Linfeng;Li, Qi;Xiao, Anwen;Li, Chonghua;Li, Yichun
关键词：Soil amendment; Soil properties; Cd phytoavailability; Cd fractions; Bacterial community
-
Characterization of lipids in Guangdong small-ear spotted pig and their crossbreeds during cold storage revealed by LC-MS-based method
作者：Huang, Wenyi;Li, Linfeng;Wang, Pan;Liu, Huilin;Liu, Midi;Meng, Fanming;Cheng, Leiyan;Hu, Bin;Zeng, Jianhua;Yang, Linfang
关键词：Pork; Cold storage; Lipidomics; Differential lipids; Meat quality
-
Analysis of Transcriptome Differences Between Subcutaneous and Intramuscular Adipose Tissue of Tibetan Pigs
作者：Li, Xinming;Huang, Qiuyan;Meng, Fanming;Hong, Chun;Li, Baohong;Yang, Yecheng;Qu, Zixiao;Wu, Junda;Li, Fei;Xin, Haiyun;Hu, Bin;Wu, Jie;Du, Zongliang;Wang, Sutian;Huang, Qiuyan;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng
关键词：Tibetan pig; fat deposition; subcutaneous fat; intramuscular fat; RNA-seq
-
A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle
作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang
关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis
-
The Impact of FBN1-α5β1 Axis in Fibro/Adipogenic Progenitor Cells (FAPCD9-) on Intramuscular Fat Content in Pigs
作者：Tong, Xian;Zhu, Qi;Duo, Tianqi;Liang, Ziyun;Zhang, Chong;Cai, Shufang;Wang, Xiaoyu;Liu, Yihao;Li, Yongpeng;Liu, Xiaohong;He, Zuyong;Chen, Yaosheng;Mo, Delin;Hu, Bin;Zeng, Jianhua
关键词：intramuscular fat; scRNA-seq; pig; Fibro/Adipogenic Progenitor Cells; FBN1-Integrin alpha 5 beta 1axis
-
Soil conditioners promote the formation of Fe-bound organic carbon and its stability
作者：Li, Qi;Li, Linfeng;Lin, Xiaoyang;Li, Yichun;Li, Qi;Li, Linfeng;Lin, Xiaoyang;Li, Yichun;Li, Qi;Li, Linfeng;Lin, Xiaoyang;Li, Yichun;Du, Huihui
关键词：Soil conditioner; Fe-bound organic carbon; Organic carbon stability; SR-FTIR
