Th1 and Th17 mucosal immune responses elicited by nasally inoculation in mice with virulence factors of Mycoplasma hyopneumoniae
文献类型： 外文期刊
作者： Xu, Lulu 1 ; Hao, Fei 2 ; Wang, Jingjing 1 ; Feng, Zhixin 2 ; Zhang, Lei 2 ; Yuan, Ting 2 ; Chen, Rong 2 ; Zhang, Zhenzhen 2 ; Shao, Guoqing 2 ; Xiong, Qiyan 2 ; Lin, Johnson 3 ; Xie, Xing 1 ; Liu, Yongjie 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Joint Int Res Lab Anim Hlth & Food Safety, 210095, Nanjing, PR, Brazil
2.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Bioprod Engn, Minist Agr & Rural Affairs, Nanjing 210014, Peoples R China
3.Univ KwaZulu Natal Westville Campus, Coll Agr Engn & Sci, Sch Life Sci, Discipline Microbiol, Private Bag X 54001, ZA-4000 Durban, South Africa
关键词： Mycoplasma hyopneumoniae; Nicotinamide Adenine Dinucleotide-Dependent; (NADH) oxidoreductase; NADH oxidase; Intramuscular immunization; Intranasal immunization
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.848； 五年影响因子：3.957 ）
ISSN： 0882-4010
年卷期： 2022 年 172 卷
页码：
收录情况： SCI
摘要： Nicotinamide Adenine Dinucleotide-Dependent (NADH) flavin oxidoreductase and NADH oxidase (NOX) are important virulence factors of Mycoplasma hyopneumoniae (Mhp), which are devoted to the function of adhesion, oxidative stress damage and apoptosis to host cells in our previous studies. Here, immune responses of NADH flavin oxidoreductase (NFOR) and NOX in mice and immune efficacy inoculated with intramuscular (IM), intranasal (IN), intramuscular unite intranasal (IM + IN) approaches were evaluated and compared. Cellular immunity levels, systemic immune and local mucosal immune responses were investigated by indirect enzyme -linked immunosorbent assay (iELISA) and quantitative reverse transcription PCR (qRT-PCR). Mice inoculated with NFOR and NOX by IM and IN or IM + IN could induce obvious secretion of specific immunoglobulin G (IgG) and secretory immunoglobulin A antibodies (sIgA) compared to those in negative control group. IM + IN inoculation resulted in systemic and local mucosal immune responses that were strongly produced. Moreover, Mhp NFOR and NOX could activate local mucosal immune responses mediated by Th1 and Th17 cells by IN. Our finding supported the notion that IM + IN was an effective immunization route for Mhp, which lays a foundation for more effective prevention of Mhp, and provides theoretical basis for the development of new subunit vaccines of Mhp.
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