Membrane vesicles derived from Streptococcus suis serotype 2 induce cell pyroptosis in endothelial cells via the NLRP3/Caspase-1/ GSDMD pathway
文献类型： 外文期刊
作者： Shi, Keda 1 ; Li, Yan 1 ; Xu, Minsheng 1 ; Zhang, Kunli 1 ; Gou, Hongchao 1 ; Li, Chunling 1 ; Zhai, Shaolun 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Inst Anim Hlth, Guangdong Acad Agr Sci,Sci Observat & Expt Stn Vet, Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China
2.Guangdong Lab Lingnan Modern Agr Sci & Technol, Maoming Branch, Maoming 525000, Peoples R China
3.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China
关键词： Streptococcus suis serotype 2; membrane vesicles; endocytosis; pyroptosis; NLRP3 inflammasomes; mitochondrial damage; endothelial cell
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 2022影响因子：4.8； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2024 年 23 卷 4 期
收录情况： SCI
摘要： Streptococcus suis serotype 2 ( S . suis 2) is a zoonotic pathogen that clinically causes severe swine and human infections (such as meningitis, endocarditis, and septicemia). In order to cause widespread diseases in different organs, S . suis 2 must colonize the host, break the blood barrier, and cause exaggerated inflammation. In the last few years, most studies have focused on a single virulence factor and its influences on the host. Membrane vesicles (MVs) can be actively secreted into the extracellular environment contributing to bacteria -host interactions. Gramnegative bacteria -derived outer membrane vesicles (OMVs) were recently shown to activate host Caspase-11mediated non -canonical inflammasome pathway via deliverance of OMV-bound lipopolysaccharide (LPS), causing host cell pyroptosis. However, little is known about the effect of the MVs from S . suis 2 (Gram-positive bacteria without LPS) on cell pyroptosis. Thus, we investigated the molecular mechanism by which S . suis 2 MVs participate in endothelial cell pyroptosis. In this study, we used proteomics, electron scanning microscopy, fluorescence microscope, Western blotting, and bioassays, to investigate the MVs secreted by S . suis 2. First, we demonstrated that S . suis 2 secreted MVs with an average diameter of 72.04 nm, and 200 proteins in MVs were identified. Then, we showed that MVs were transported to cells via mainly dynamin-dependent endocytosis. The S . suis 2 MVs activated NLRP3/Caspase-1/GSDMD canonical inflammasome signaling pathway, resulting in cell pyroptosis, but it did not activate the Caspase-4/-5 pathway. More importantly, endothelial cells produce large amounts of reactive oxygen species (ROS) and lost their mitochondrial membrane potential under induction by S . suis 2 MVs. The results in this study suggest for the first time that MVs from S . suis 2 were internalized by endothelial cells via mainly dynamin-dependent endocytosis and might promote NLRP3/Caspase-1/GSDMD pathway by mitochondrial damage, which produced mtDNA and ROS under induction, leading to the pyroptosis of endothelial cells.
- 相关文献
作者其他论文 更多>>
-
Novel perspectives on autophagy-oxidative stress-inflammation axis in the orchestration of adipogenesis
作者：Hong, Chun;Li, Xinming;Huang, Qiuyan;Li, Baohong;Xin, Haiyun;Hu, Bin;Meng, Fanming;Li, Jianhao;Wang, Sutian;Zhang, Kunli;Huang, Qiuyan;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng;Hu, Chuanhuo;Tao, Chenyu;Cao, Yang;Wang, Hai;Deng, Bo;Wang, Sutian
关键词：adipogenesis; autophagy; inflammation; oxidative stress; immune responses
-
The cGAS-STING pathway in viral infections: a promising link between inflammation, oxidative stress and autophagy
作者：Zhang, Kunli;Zhao, Ziqiao;Sun, Zeyi;Li, Chunling;Zhang, Jianfeng;Huang, Qiuyan;Li, Xinming;Hong, Chun;Wang, Sutian;Huang, Qiuyan;Deng, Bo;Zhang, Jianfeng;Wang, Sutian
关键词：cGAS-STING; viral infection; innate immune; autophagy; inflammation; oxidative stress; virus-host interaction
-
TLR4 signalling in ischemia/reperfusion injury: a promising target for linking inflammation, oxidative stress and programmed cell death to improve organ transplantation outcomes
作者：Wang, Sutian;Huang, Qiuyan;Meng, Fanming;Zhang, Kunli;Deng, Shoulong;Deng, Shoulong
关键词：toll-like receptor 4; ischemia/reperfusion injury; organ transplantation; inflammation; oxidative stress; programmed cell death
-
Display of porcine epidemic diarrhea virus spike protein B-cell linear epitope on Lactobacillus mucosae G01 S-layer surface induce a robust immunogenicity in mice
作者：Zhang, Bin;Liu, Zhicheng;Wuri, Nile;Geri, Letu;Zhang, Bin;Gou, Hongchao;Shen, Haiyan;Zhang, Chunhong;Liu, Zhicheng;Wuri, Nile;Nie, Jingjing;Qu, Yunzhi;Zhang, Jianfeng
关键词：PEDV; Epitope; SLP; L. mucosae G01; Probiotics surface
-
A novel linear displacement isothermal amplification with strand displacement probes (LDIA-SD) in a pocket-size device for point-of-care testing of infectious diseases
作者：Gou, Hongchao;Shen, Haiyan;Zhang, Chunhong;Liao, Ming;Lin, Qijie;Jia, Kaiyuan;Liang, Yucen;Peng, Junhao;Qu, Xiaoyun;Zhang, Jianmin;Liao, Ming;Li, Yanbin;Lin, Jianhan;Zhang, Jianmin;Liao, Ming
关键词：Nucleic Acid Amplification; Isothermal amplification; Point -of -care testing (POCT); Linear displacement isothermal amplification; (LDIA); Infectious diseases
-
TLR4 Overexpression Aggravates Bacterial Lipopolysaccharide-Induced Apoptosis via Excessive Autophagy and NF-& kappa;B/MAPK Signaling in Transgenic Mammal Models
作者：Wang, Sutian;Huang, Qiuyan;Yang, Yecheng;Meng, Fanming;Li, Jianhao;Luo, Chenglong;Zhang, Kunli;Song, Xuting;Lu, Qi;Zhao, Duowei;Yao, Yuchang;Lin, Sen;Deng, Shoulong;Deng, Shoulong;Qi, Meiyu;Lian, Zhengxing
关键词：Toll-like receptor 4; transgenic animal model; autophagy; apoptosis; inflammation; oxidative stress
-
Development of a multiplex qRT-PCR assay for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine Deltacoronavirus
作者：Li, Yan;Niu, Jia-Wei;Zhou, Xia;Chu, Pin-Pin;Zhang, Kun-Li;Gou, Hong-Chao;Yang, Dong-Xia;Zhang, Jian-Feng;Li, Chun-Ling;Liao, Ming;Zhai, Shao-Lun;Liao, Ming
关键词：porcine epidemic diarrhea virus; Transmissible Gastroenteritis Virus; Porcine Deltacoronavirus; porcine coronaviruses; multiplex real-time qRT-PCR
