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Membrane vesicles derived from Streptococcus suis serotype 2 induce cell pyroptosis in endothelial cells via the NLRP3/Caspase-1/ GSDMD pathway

文献类型： 外文期刊

作者： Shi, Keda 1 ; Li, Yan 1 ; Xu, Minsheng 1 ; Zhang, Kunli 1 ; Gou, Hongchao 1 ; Li, Chunling 1 ; Zhai, Shaolun 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Inst Anim Hlth, Guangdong Acad Agr Sci,Sci Observat & Expt Stn Vet, Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China

2.Guangdong Lab Lingnan Modern Agr Sci & Technol, Maoming Branch, Maoming 525000, Peoples R China

3.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China

关键词： Streptococcus suis serotype 2; membrane vesicles; endocytosis; pyroptosis; NLRP3 inflammasomes; mitochondrial damage; endothelial cell

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2024 年 23 卷 4 期

页码：

收录情况： SCI

摘要： Streptococcus suis serotype 2 ( S . suis 2) is a zoonotic pathogen that clinically causes severe swine and human infections (such as meningitis, endocarditis, and septicemia). In order to cause widespread diseases in different organs, S . suis 2 must colonize the host, break the blood barrier, and cause exaggerated inflammation. In the last few years, most studies have focused on a single virulence factor and its influences on the host. Membrane vesicles (MVs) can be actively secreted into the extracellular environment contributing to bacteria -host interactions. Gramnegative bacteria -derived outer membrane vesicles (OMVs) were recently shown to activate host Caspase-11mediated non -canonical inflammasome pathway via deliverance of OMV-bound lipopolysaccharide (LPS), causing host cell pyroptosis. However, little is known about the effect of the MVs from S . suis 2 (Gram-positive bacteria without LPS) on cell pyroptosis. Thus, we investigated the molecular mechanism by which S . suis 2 MVs participate in endothelial cell pyroptosis. In this study, we used proteomics, electron scanning microscopy, fluorescence microscope, Western blotting, and bioassays, to investigate the MVs secreted by S . suis 2. First, we demonstrated that S . suis 2 secreted MVs with an average diameter of 72.04 nm, and 200 proteins in MVs were identified. Then, we showed that MVs were transported to cells via mainly dynamin-dependent endocytosis. The S . suis 2 MVs activated NLRP3/Caspase-1/GSDMD canonical inflammasome signaling pathway, resulting in cell pyroptosis, but it did not activate the Caspase-4/-5 pathway. More importantly, endothelial cells produce large amounts of reactive oxygen species (ROS) and lost their mitochondrial membrane potential under induction by S . suis 2 MVs. The results in this study suggest for the first time that MVs from S . suis 2 were internalized by endothelial cells via mainly dynamin-dependent endocytosis and might promote NLRP3/Caspase-1/GSDMD pathway by mitochondrial damage, which produced mtDNA and ROS under induction, leading to the pyroptosis of endothelial cells.

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