Functional properties of glutelin from Camellia oleifera seed cake: Improvement by alkali-assisted phosphorylation through changes in protein structure
文献类型： 外文期刊
作者： Yu, Ningxiang 1 ; Wang, Yijue 1 ; Shao, Shengxin 1 ; Li, Jie 2 ; Li, Mengren 2 ; Zhu, Lizhong 2 ; Ye, Qin 3 ; Huan, Weiwei 2 ; Meng, Xianghe 1 ;
作者机构： 1.Zhejiang Univ Technol, Coll Food Sci & Technol, Hangzhou 310014, Zhejiang, Peoples R China
2.Zhejiang A&F Univ, Coll Chem & Mat Engn, Hangzhou 311300, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310014, Zhejiang, Peoples R China
关键词： Camellia oleifera seed cake; Glutelin; Phosphorylation; Aggregation; Solubility; In vitro digestibility
期刊名称：CURRENT RESEARCH IN FOOD SCIENCE （ 影响因子：6.3； 五年影响因子：6.3 ）
ISSN：
年卷期： 2023 年 6 卷
页码：
收录情况： SCI
摘要： To explore the effect and its mechanism of alkali-assisted phosphorylation on the functional properties of Camellia Oleifera seeds cake glutelin (CSCG), CSCG was treated with different concentration of sodium trimetaphosphate (STMP, 1.0, 2.0, 3.0, 4.0, and 5%, w/v) in different pH environment (3.0, 5.0, 7.0, 9.0, and 11.0). The results showed that alkali assist improved the phosphorylation degree of CSCG, and the optimum pH value is 9.0. FT-IR and XPS confirmed the successful modification of phosphate groups on CSCG through covalent interaction. Alkali-assisted phosphorylation decreased the particle size and increased electronegativity of CSCG, as well as changed in its surface hydrophobicity, crystallinity, and intrinsic fluorescence. All these changes of protein structure triggered by alkali-assisted phosphorylation led to the improvement of water solubility, water/ oil absorption capacity, emulsifying ability, foamability, and in vitro digestibility of CSCG. This work could provide a theoretical basis for industrial production of CSCG with excellent functional properties.
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