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Detection of soybean transgenic event GTS-40-3-2 using electric field-induced release and measurement (EFIRM)

文献类型： 外文期刊

作者： Zheng, Qiuyue 1 ; Ji, Chao 2 ; Liu, Ran 4 ; Xu, Junyi 4 ; Wang, Yong 5 ; Yang, Aifu 4 ; Zheng, Wenjie 2 ; Cao, Jijuan 1 ;

作者机构： 1.Dalian Minzu Univ, Coll Life Sci, Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China

2.Tianjin Normal Univ, Lab Qual Control & Traceabil Food, Tianjin 300387, Peoples R China

3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650201, Yunnan, Peoples R China

4.Technol Ctr Dalian Customs Dist, Dalian 116001, Peoples R China

5.Tianjin Acad Agr Sci, Tianjin 300192, Peoples R China

关键词： Transgenic soybean; Genetically modified organisms (GMOs); GTS-40-3-2; Detection; Electric field-induced release and measurement (EFIRM)

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.157； 五年影响因子：3.868 ）

ISSN： 1618-2642

年卷期： 2021 年 413 卷 26 期

页码：

收录情况： SCI

摘要： Polymerase chain reaction (PCR) technology has become a standard technique for the detection of genetically modified organisms (GMOs). However, this method requires a PCR amplification process which is both expensive and time-consuming. Herein, we propose electric field-induced release and measurement (EFIRM) technology as an alternative method for GMO screening. The specificity and sensitivity of the EFIRM assay were proven to be comparable to those of the real-time PCR method for detecting genetically modified soybeans. After all the parameters had been evaluated, the actual evaluation of soybean samples from soybean cargoes was performed. An actual EFIRM screening was performed on 157 soybean cargo samples, which had 102 transgenic soybean samples containing the GTS-40-3-2 gene, through a blind trial at the Dalian port of China. Our results showed that 101 transgenic soybean samples were correctly detected, with only one false-negative case, and 55 non-transgenic soybean samples were detected as negative; this demonstrates that the EFIRM assay is an effective, accurate, simple, and economical novel method for detecting transgenic products, which may have a positive impact on the development of rapid on-site GMO monitoring platforms.

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