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Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection

文献类型： 外文期刊

作者： Sun, Zhaodi 1 ; Ren, Yuqin 4 ; Zhang, Yitong 4 ; Yang, Yucong 1 ; Wang, Guixing 4 ; He, Zhongwei 4 ; Liu, Yufeng 4 ; Cao, Wei 4 ; Wang, Yufen 4 ; Fu, Yuanshuai 1 ; Hou, Jilun 4 ;

作者机构： 1.Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China

2.Shanghai Collaborat Innovat Aquat Anim Genet & Bre, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Lab Cell & Mol Biol, Shanghai 201306, Peoples R China

4.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China

关键词： Paralichthys olivaceus; Cell line; Bohle virus; Chinese giant salamander iridovirus

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2023 年 562 卷

页码：

收录情况： SCI

摘要： Fish cell lines are important tools in many biological studies. Here, we established a new Japanese flounder brain (JFB) cell line from the brain tissue of the Japanese flounder (Paralichthys olivaceus), which was successfully sub-cultured for over 120 passages, and consisted predominantly of fibroblast-like cells. Sequencing of the actin gene confirmed that JFB cells were derived from the Japanese flounder, and JFB was also identified as a neural stem cell line on the basis of the mRNA expression of nestin gene. The JFB cells could grow at a temperature ranging from 17 to 29 degrees C, and 23 degrees C was the optimum temperature. Karyotype analysis showed that the chromosome mode number was 48, demonstrating a normal diploid chromosome number. The transfection efficiency of pEGFP-N1 in JFB cells was up to 30%. The JFB cells showed susceptibility to Hirame rhabdovirus (HIRRV), Lymphocystis disease virus (LCDV), Bohle virus (BIV) and Chinese giant salamander iridovirus (GSIV), which was demonstrated by varying degrees of cytopathic effects. Viral infection or Poly (I:C) stimulation upregulated the expression of multiple immune-related genes, including IL-1 beta, IL8, Mx and TNF. These results showed that the newly established JFB cell line is an ideal tool for studying gene manipulation, host-virus interaction and po-tential vaccines development.

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