tsRNA-00764 Regulates Estrogen and Progesterone Synthesis and Lipid Deposition by Targeting PPAR-γ in Duck Granulosa Cells
文献类型： 外文期刊
作者： Chen, Yaru 1 ; Wu, Yan 1 ; Pi, Jinsong 1 ; Fu, Ming 1 ; Shen, Jie 1 ; Zhang, Hao 1 ; Du, Jinping 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Wuhan 430064, Peoples R China
2.Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China
关键词： transfer RNA-derived small RNAs (tsRNAs); follicle selection; duck granulosa cells; progesterone synthesis; estrogen synthesis; lipid deposition
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 20 期
页码：
收录情况： SCI
摘要： Transfer RNA-derived small RNAs (tsRNAs) are novel regulatory small non-coding RNAs that have been found to modulate many life activities in recent years. However, the exact functions of tsRNAs in follicle development remain unclear. Follicle development is a remarkably complex process that follows a strict hierarchy and is strongly associated with reproductive performance in ducks. The process of converting small yellow follicles into hierarchal follicles is known as follicle selection, which directly determines the number of mature follicles. We performed small RNA sequencing during follicle selection in ducks and identified tsRNA-00764 as the target of interest based on tsRNA expression profiles in this study. Bioinformatics analyses and luciferase reporter assays further revealed that peroxisome proliferator-activated receptor-gamma (PPAR-gamma) was the target gene of tsRNA-00764. Moreover, tsRNA-00764 knockdown promoted estrogen and progesterone synthesis and lipid deposition in duck granulosa cells, while a PPAR-gamma inhibitor reversed the above phenomenon. Taken together, these results demonstrate that tsRNA-00764, differentially expressed in pre-hierarchal and hierarchy follicles, modulates estrogen and progesterone synthesis and lipid deposition by targeting PPAR-gamma in duck granulosa cells, serving as a potential novel mechanism of follicle selection. Overall, our findings provide a theoretical foundation for further exploration of the molecular mechanisms underlying follicle development and production performance in ducks.
- 相关文献
作者其他论文 更多>>
-
Transcriptomic insight into the underlying mechanism of induced molting on reproductive remodeling, performance and egg quality in laying hen
作者：Ma, Pengyun;Chen, Jilan;Zhang, Xiaoke;Xu, Xinying;Ma, Zhong;Li, Yunlei;Ma, Pengyun;Xue, Fuguang;Zhang, Hao;Wu, Yan;Li, Ling;Qu, Yuanqi
关键词：Induced molting; Reproductive remodeling; Egg quality; Transcriptomic analysis; Laying hen
-
New Insights into the Regulatory Non-Coding RNAs Mediating Rice-Brown Planthopper Interactions
作者：Hu, Liang;Wu, Yan;Zha, Wenjun;Zhou, Lei;You, Aiqing;Zhou, Lei;You, Aiqing
关键词：rice; brown planthopper; non-coding RNA; resistance; cross-kingdom interactions
-
Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs
作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang
关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits
-
Wheat Stripe Rust Inoculum from the Southwest Dispersed to the East Huang-Huai-Hai Region Through Southern Anhui in China
作者：Huang, Liang;Zhao, Wuchao;Xia, Chongjing;Zhao, Na;Li, Hongfu;Zhang, Hao;Chen, Wanquan;Liu, Taiguo;Huang, Liang;Xia, Chongjing;Zhao, Na;Li, Hongfu;Sun, Zhenyu;Zhang, Hao;Chen, Wanquan;Liu, Taiguo;Zhao, Wuchao;Sun, Zhenyu;Yang, Lijun;Li, Mingju;Chen, Wen;Yang, Fang
关键词：dispersal pathway; inoculum source; southwest of Anhui; wheat stripe rust
-
Advances in Anther Culture-Based Rice Breeding in China
作者：Chen, Xinxing;Li, Sanhe;Zha, Wenjun;Li, Changyan;Zhou, Lei;You, Aiqing;Wu, Yan;Chen, Xinxing;Zhou, Lei;You, Aiqing
关键词：rice (
Oryza sativa L.) anther culture breeding; influencing factors; current status of research; prospect -
Regulatory effects of the bidirectional crosstalk between hepatic gene expressions and gut microbiota on the productive performances and egg quality of induced molting treated layer hens
作者：Xue, Fuguang;Ma, Pengyun;Li, Yunlei;Zhang, Xiaoke;Xu, Xinying;Ma, Zhong;Sun, Yanyan;Yuan, Jingwei;Ma, Hui;Chen, Jilan;Xue, Fuguang;Ma, Pengyun;Wu, Huadong;Pi, Jinsong;Li, Ling;Qu, Yuanqi
关键词：Fasting-induced molting; Egg quality; Gut microbiota; Hepatic gene expression; Layer hens
-
RNA-seq Analysis of the Mechanisms Underlying Chalky Grain and Characterization of Two IAA Receptor Proteins OsAFB3 and OsAFB5 in Chalkiness Formation in Oryza sativa
作者：Shi, Shaojie;Wang, Huiying;Zha, Wenjun;Huang, An;Chen, Ziyi;Wu, Yan;Chen, Junxiao;Li, Changyan;Wu, Bian;Li, Sanhe;Xu, Huashan;Li, Peide;Liu, Kai;Chen, Zhijun;Yang, Guocai;Zhou, Lei;You, Aiqing;Zhou, Lei;You, Aiqing;Huang, An;Chen, Ziyi
关键词：Rice; Chalkiness; Grain quality; IAA signal transduction; Breeding
