Characterization of a Streptococcus species isolated from Siganus guttatus in South China
文献类型： 外文期刊
作者： Li, Lili 1 ; Wang, Chong 3 ; Olsen, Rikke Heidemann 4 ; Li, Xianfeng 2 ; Meng, Hecheng 2 ; Xu, Liwen 5 ; Shi, Lei 1 ;
作者机构： 1.Jinan Univ, Inst Food Safety & Nutr, Guangzhou, Guangdong, Peoples R China
2.South China Univ Technol, Sch Food Sci & Engn, Guangzhou, Guangdong, Peoples R China
3.Shandong New Hope Liuhe Grp Ltd, Qingdao, Peoples R China
4.Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet & Anim Sci, Frederiksberg C, Denmark
5.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou, Guangdong, Peoples R China
6.State Key Lab Food Safety Technol Meat Prod, Xiamen 361100, Peoples R China
关键词： Streptococcus; Virulence; Siganus guttatus; Detection
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2021 年 545 卷
页码：
收录情况： SCI
摘要： Streptococcus has emerged as an important pathogen in fish farming worldwide. In this study, we characterized a Streptococcus species from a cultured, diseased Siganus guttatus in South China. Phenotypic, chemotaxonomic, 16S rRNA gene sequencing, phylogenetic and genome comparison analysis indicated that this isolate may be considered as same species with previously reported Streptococcus sp. X13SY08 from Murray cod (Maccullochella peelii peelii). We further predicted the virulence factor using whole genome sequence and analyzed the pathogenesis of this species by challenge tests. A total of 30 virulence factors were identified on the genome. Challenge tests of S. guttatus via intrathoracic injection of strain X16XC17 exhibited 100% cumulative mortality rates at 10(8) colony forming units (CFU) per fish, and 45% to 85% at 10(4) to 10(7) CFU per fish. The histopathological results showed that the spleen, kidney, liver and heart all had pathological manifestations. Additionally, a specific PCR assay was developed to detect this species. The antibiotic resistance results showed that this species was only resistant to trimethoprim-sulfamethoxazole. This study describes a new species of genus Streptococcus as potential causative agent of diseased S. guttatus, and offers insights in the subsequent study of the virulence and pathogenesis associated with this pathogen.
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