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miR-2765 involved in ammonia nitrogen stress via negative regulation of autophagy in shrimp

文献类型： 外文期刊

作者： Wang, Feifei 1 ; Zhao, Fei 1 ; Deng, Yuting 1 ; Tan, Aiping 1 ; Lai, Yingtiao 1 ; Gong, Hua 1 ; Huang, Zhibin 1 ; Liu, Yuan 2 ; Liang, Qingjian 2 ; Wang, Weina 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Key Lab Fishery Drug Dev,Minist Agr & Rural Affair, Guangzhou, Guangdong, Peoples R China

2.South China Normal Univ, Coll Life Sci, Guangzhou Key Lab Subtrop Biodivers & Biomonitorin, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Peoples R China

3.Zhejiang Ocean Univ, Sch Fishery, Lab Aquat Anim Dis & Immun, Zhoushan 316022, Zhejiang, Peoples R China

4.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, 1 Xingyu Rd, Guangzhou 510380, Guangdong, Peoples R China

关键词： Pva-miR-2765; Penaeus vannamei; Ammonia nitrogen; Autophagy

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 258 卷

页码：

收录情况： SCI

摘要： MicroRNA (miRNA) is a highly conserved non-coding tiny endogenous RNA molecule that regulates various cellular functions by inhibiting mRNA translation or promoting the degradation of proteins. In this study, we identified a specific miRNA (designed as Pva-miR-2765) from Penaeus vannamei, which widely distributed in different tissues of shrimp, with the highest concentration found in the intestine. Through fluorescence in situ hybridization (FISH), we observed that Pva-miR-2765 is primarily located in the cytoplasm. Interestingly, we found that the expression of Pva-miR-2765 significantly decreased in hemocytes, hepatopancreas and gill under ammonia nitrogen stress. Furthermore, when Pva-miR-2765 was silenced, the autophagy level in shrimp significantly increased. Additionally, Pva-miR-2765 was found to promote pathological damage in the hepatopancreas of shrimp. Subsequently, correlation analysis revealed a negative relationship between the expression of Pva-miR-2765 and PvTBC1D7. To confirm this interaction, we conducted a dual luciferase reporter gene assay, which demonstrated that Pva-miR-2765 inhibit the expression of PvTBC1D7 by interacting with its 3'UTR. And the expression level of PvTBC1D7 in shrimp decreased significantly under ammonia nitrogen stress in Pva-miR2765 overexpressed. Our findings suggest that Pva-miR-2765 can reduce autophagy in P. vannamei by inhibiting the regulation of PvTBC1D7, thereby participating in the oxidative stress of shrimp caused by ammonia nitrogen stress.

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