Characterization of inulolytic enzymes from the Jerusalem artichoke-derived Glutamicibacter mishrai NJAU-1
文献类型： 外文期刊
作者： Lian, Dan 1 ; Zhuang, Shuo 1 ; Shui, Chen 1 ; Zheng, Shicheng 1 ; Ma, Yanhong 2 ; Sun, Zongjiu 3 ; Porras-Dominguez, Jaime R. 4 ; Oner, Ebru Toksoy 6 ; Liang, Mingxiang 1 ; Van den Ende, Wim 4 ;
作者机构： 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Jiangsu, Peoples R China
3.Xinjiang Agr Univ, Coll Grassland & Environm Sci, Urumqi 830052, Xinjiang, Peoples R China
4.Katholieke Univ Leuven, Lab Mol Plant Biol, Kasteelpk Arenberg 31, B-3001 Louvain, Belgium
5.Katholieke Univ Leuven, Plant Inst, Kasteelpk Arenberg 31, B-3001 Louvain, Belgium
6.Marmara Univ, Fac Engn, Dept Bioengn, TR-34722 Istanbul, Turkey
关键词： Bio fructose; Exo-inulinase; Glutamicibacter mishrai; Fructan; Jerusalem artichoke
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.56； 五年影响因子：5.365 ）
ISSN： 0175-7598
年卷期：
页码：
收录情况： SCI
摘要： The rhizosphere context of inulin-accumulating plants, such as Jerusalem artichoke (Helianthus tuberosus), is an ideal starting basis for the discovery of inulolytic enzymes with potential for bio fructose production. We isolated a Glutamicibacter mishrai NJAU-1 strain from this context, showing exo-inulinase activity, releasing fructose from fructans. The growth conditions (pH 9.0; 15 degrees C) were adjusted, and the production of inulinase by Glutamicibacter mishrai NJAU-1 increased by 90% (0.32 U/mL). Intriguingly, both levan and inulin, but not fructose and sucrose, induced the production of exo-inulinase activity. Two exo-inulinase genes (inu/ and inu2) were cloned and heterologously expressed in Pichia pastoris. While INU2 preferentially hydrolyzed longer inulins, the smallest fructan 1-kestose appeared as the preferred substrate for INU1, also efficiently degrading nystose and sucrose. Active site docking studies with GFn- and Fn-type small inulins (G is glucose, F is fructose, and n is the number of beta (2-1) bound fructose moieties) revealed subtle substrate differences between INU1 and INU2. A possible explanation about substrate specificity and INU's protein structure is then suggested.
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