Facile and green synthesis of reduced graphene oxide/loofah sponge for Streptomyces albulus immobilization and e-poly-L-lysine production
文献类型： 外文期刊
作者： Wang, Cheng 1 ; Chen, Xi 4 ; Jiang, Yingying 3 ; Li, Na 1 ; Zhu, Ping 2 ; Xu, Hong 1 ;
作者机构： 1.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
3.Natl Univ Singapore, Dept Phys, Singapore 117551, Singapore
4.Nanjing Shangqin New Mat Technol Co, Nanjing 210046, Peoples R China
关键词： Graphene oxide; Loofah sponge; Cell immobilization; Streptomyces albulus; e-Poly-L-lysine; Aerobic plant fibrous-bed bioreactor
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.889； 五年影响因子：11.139 ）
ISSN： 0960-8524
年卷期： 2022 年 349 卷
页码：
收录情况： SCI
摘要： Facile and green fabrication of reduced graphene oxide on loofah sponge (rGOLS) carrier was applied for cell immobilization of e-Poly -L-lysine (epsilon-PL) production. Due to surface properties including large specific surface area, high polarity, and low interaction energy, rGOLS-1 was employed as the optimum rGOLS to enhance immobilization of Streptomyces albulus. Compared with raw LS, batch experiments showed rGOLS-1 facilitated superior cell vitality for epsilon-PL production due to the presence of reduced graphene oxide. In the sequential fed batch cultivation of Streptomyces albulus using rGOLS-1 with an aerobic plant fibrous-bed bioreactor (rGOLS-1AFPB), the maximum epsilon-PL concentration and productivity reached to 39.2 +/- 0.63 g/L and 0.48 g/L/h. The cells immobilized in rGOLS-1 with high vitality and epsilon-PL production efficiency were reused six times over a period of 624 h. This research afforded an effective approach to enhance the fermentation performance of immobilized cells with the design of an advanced immobilization carrier.
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