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Near-infrared reflectance Spectroscopy-based methods for phytase registration in feed industry

文献类型： 外文期刊

作者： Yang, Haifeng 1 ; Lv, Xiaowen 2 ; Wang, Jing 2 ; Li, Junguo 2 ; Li, Hui 2 ; Qin, Yuchang;

作者机构： 1.Chinese Acad Agr Sci, Feed Res inst, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Feed Res inst, Beijing 100081, Peoples R China; Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100080, Peoples R China; Shanghai Acad Agr Sci, Inst Agrifood Standards & Testing Technol, Shanghai, Peoples R China

关键词： near-infrared reflectance spectroscopy;phytase;registration;feed control;ESCHERICHIA-COLI;ACID-PHOSPHATASE;NIRS;PURIFICATION;IDENTIFICATION;PRINCIPLES;BIOPROCESS;QUALITY;MEAL

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The application of biotechnological products in the feed industry has undergone explosive growth in recent years, and phytase from microorganism accounts for one-third of the entire feed enzyme market. In this study, some differences in the composition of protein and denaturation temperature between two commercial phytases were determined by HPLC and differential scanning calorimetry, which were derived from the same origin of E. coli. At the same time, we found that it was advantageous for near-infrared reflectance spectroscopy (NIRS) to display the protein differences in the commercial phytase, which is most important for ensuring the traceability of biotechnological products in feed and food safety control. Furthermore, NIRS could track the changes in phytase during the spray-drying process and the change of enzyme activity during storage of phytase. Our experiments proved that the information from NIRS could describe well the individual characteristics of the commercial phytase, which indicated that near-infrared reflectance spectra could be exploited to use in the registration system of commercial phytase.

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