Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation
文献类型： 外文期刊
作者： Hu, Bowen 1 ; Zhao, Changbin 1 ; Pan, Xiangchun 4 ; Wei, Haohui 1 ; Mo, Guodong 1 ; Xian, Mingjian 1 ; Luo, Wen 1 ; Nie, Qinghua 1 ; Li, Hongmei 1 ; Zhang, Xiquan 1 ;
作者机构： 1.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Guangzhou, Guangdong, Peoples R China
2.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Guangdong, Peoples R China
3.South China Agr Univ, Lingnan Guangdong Lab Agr, Guangzhou, Guangdong, Peoples R China
4.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China
关键词： GHR; IGF1; Mitochondrial biogenesis; Mitochondrial function; Myoblast differentiation
期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.4； 五年影响因子：8.0 ）
ISSN：
年卷期： 2023 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Background Myoblast differentiation requires metabolic reprogramming driven by increased mitochondrial biogenesis and oxidative phosphorylation. The canonical GH-GHR-IGFs axis in liver exhibits a great complexity in response to somatic growth. However, the underlying mechanism of whether local GHR acts as a control valve to regulate mitochondrial function through mitochondrial biogenesis during myoblast differentiation remains unknown. Methods We manipulated the GHR expression in chicken primary myoblast to investigate its roles in mitochondrial biogenesis and function during myoblast differentiation. Results We reported that GHR is induced during myoblast differentiation. Local GHR promoted mitochondrial biogenesis during myoblast differentiation, as determined by the fluorescence intensity of Mito-Tracker Green staining and MitoTimer reporter system, the expression of mitochondrial biogenesis markers (PGC1 alpha, NRF1, TFAM) and mtDNA encoded gene (ND1, CYTB, COX1, ATP6), as well as mtDNA content. Consistently, local GHR enhanced mitochondrial function during myoblast differentiation, as determined by the oxygen consumption rate, mitochondrial membrane potential, ATP level and ROS production. We next revealed that the regulation of mitochondrial biogenesis and function by GHR depends on IGF1. In terms of the underlying mechanism, we demonstrated that IGF1 regulates mitochondrial biogenesis via PI3K/AKT/CREB pathway. Additionally, GHR knockdown repressed myoblast differentiation. Conclusions In conclusion, our data corroborate that local GHR acts as a control valve to enhance mitochondrial function by promoting mitochondrial biogenesis via IGF1-PI3K/AKT/CREB pathway during myoblast differentiation.
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