Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber
文献类型： 外文期刊
作者： Li, Yanan 1 ; Yan, Lei 2 ; Kong, Xue 4 ; Chen, Jiawei 1 ; Zhang, Haibin 1 ;
作者机构： 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Open Sea Fishery Dev, Minist Agr & Rural Affairs Peoples Republ China, Guangzhou 510300, Peoples R China
3.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China
4.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Ecoenvironm, Lianyungang 222005, Peoples R China
关键词： Purification; Deep-sea enzyme; The Mariana trench
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 163 卷
页码：
收录情况： SCI
摘要： Superoxide dismutases (SODs) are the first line of defense against oxidative damage caused by reactive oxygen species and are associated with stress tolerance. Accordingly, many studies have focused on SODs, especially those from extreme habitats. In this study, we reported a novel Cu,Zn-SOD from the new species Benthodytes marianensis sp. nov (hereafter denoted as Bm-Cu,Zn-SOD) collected from the Mariana Trench. The purified recombinant enzyme with an approx. Mr. of 38 kDa was intracellular and dimeric, and it expressed activity at an optimal temperature of 30 degrees C and optimum pH of 7.4. The Km and Vmax values of Bm-Cu,Zn-SOD were 0.046 +/- 0.004 mM and 1209.151 +/- 24.057 U/mg, respectively. Under the tested conditions, Bm-Cu,Zn-SOD resisted interference from chemicals (beta-mercaptoethanol and ethylenediaminetetraacetic acid), denaturants (urea and guanidine hydrochloride), and high salinity, showing high kinetic stability. Furthermore, Bm-Cu,Zn-SOD could be activated by high hydrostatic pressure. All these properties suggested adaptation to the deep-sea environment and thus indicated its potential future applications. (C) 2020 Elsevier B.V. All rights reserved.
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