The Globodera pallida Effector GpPDI1 Is a Functional Thioredoxin and Triggers Defense-Related Cell Death Independent of Its Enzymatic Activity
文献类型： 外文期刊
作者： Gross, Rachel 1 ; Zhang, Shan 1 ; Wei, Lihui 3 ; Caplan, Allan 1 ; Kuhl, Joseph 1 ; Dandurand, Louise-Marie 4 ; Wang, 1 ;
作者机构： 1.Univ Idaho, Dept Plant Sci, Moscow, ID 83843 USA
2.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
4.Univ Idaho, Dept Entomol Plant Pathol & Nematol, Moscow, ID 83843 USA
期刊名称：PHYTOPATHOLOGY （ 影响因子：4.025； 五年影响因子：4.394 ）
ISSN： 0031-949X
年卷期： 2020 年 110 卷 11 期
页码：
收录情况： SCI
摘要： The plant-parasitic nematode Globodera pallida is an obligate biotroph that only reproduces on select species in the Solanum family. The establishment of the feeding site, the syncytium, involves secretion of effectors into the plant cell to combat the plant defense response and facilitate transformation of root cells into the syncytium. Despite the important predicted roles of effectors in the plant_ pathogen interactions, the functionality of G. pallida effectors is largely unknown. In this study, we identified and characterized a G. pallida effector protein disulfide isomerase (GpPDI1). GpPDI1 contains two thioredoxin domains that function together to reduce disulfide bonds, as manifested by the nullification of enzymatic activity when either domain is absent. The transcript of GpPDI1 is localized in the dorsal gland of the nematode during the J2 stage. In addition, GpPDI1 can trigger defense- related cell death in Nicotiana benthamiana and tomato (Solanum lycopersicum) leaf tissue and localizes in the plant host cell's cytoplasm and nucleus when transiently expressed in plant cells. Significantly, the ability of elicitation of cell death is not dependent on the enzymatic activity of GpPDI1 or correlated with the subcellular distribution of GpPDI1, suggesting that a nondisulfide reducing function or structural feature of GpPDI1 is responsible for the recognition by the host immune system to elicit cell death.
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