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Application of recombinase polymerase amplification method for rapid detection of infectious laryngotracheitis virus

文献类型： 外文期刊

作者： Zhu, Yujun 1 ; Zeng, Fanwen 3 ; Sun, Junying 4 ; Liu, Xiangnan 5 ; Wu, Miaoli 1 ; Huang, Bihong 1 ; Lian, Yuexiao 1 ; Xia 1 ;

作者机构： 1.Anim Monitoring Inst, Guangdong Lab, Guangzhou 510633, Peoples R China

2.Guangdong Prov Key Lab, Lab Anim, Guangzhou 510633, Peoples R China

3.South China Agr Univ, Coll Anim Sci, Wushan Rd 483, Guangzhou 510642, Peoples R China

4.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Open Lab Vet Publ Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou 510640, Guangdong, Peoples R China

5.Guangdong Polytech Sci & Trade, Coll Anim Sci & Technol, Guangzhou 510640, Peoples R China

关键词： Infectious laryngotracheitis virus; Real-time; Recombinase polymerase amplification; Detection

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2020 年 54 卷

页码：

收录情况： SCI

摘要： Infectious laryngotracheitis is a significant respiratory disease of chickens that causes huge economic losses due to high morbidity and mortality and reduced egg production. A real-time recombinase polymerase amplification (RPA) assay was developed to accurately detect ILTV. The specific probe and primer sets were carefully designed and screened. The real-time RPA assay was carried out at 39 degrees C for 30 min, and results were obtained within 15 min. The results of the specificity assay showed no fluorescence signals with other avian-related viruses. The sensitivity of the assay was 1 x 10(2) copies/mu L. The low CV value showed that the assay was reproducible. A total of 115 clinical samples were tested using the real-time RPA assay and the real-time PCR assay in parallel; the coincidence rates of the two detection methods were 100%. The results indicated that the real-time RPA assay is a specific, sensitive, rapid, and useful tool for epidemiological studies and clinical diagnosis, especially in the field and in resource-poor areas.

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