Effects of Florfenicol on nirS-Type Denitrification Community Structure of Sediments in an Aquatic Microcosm Model
文献类型： 外文期刊
作者： Zhang, Tengyue 1 ; Sun, Junying 3 ; Peng, Jinju 1 ; Ding, Yuexia 1 ; Li, Yang 1 ; Ma, Haotian 1 ; Yu, Mengbo 1 ; Ma, Yi 1 ;
作者机构： 1.Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Vet Med, Zhanjiang 524088, Peoples R China
2.Yangzhou Univ, Coll Vet Med, Yangzhou 225000, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China
关键词： florfenicol; nirS gene; denitrification community structure; sediment; aquatic microcosm
期刊名称：ANTIBIOTICS-BASEL （ 影响因子：4.8； 五年影响因子：4.9 ）
ISSN： 2079-6382
年卷期： 2023 年 12 卷 8 期
页码：
收录情况： SCI
摘要： Florfenicol is one of the most widely used antibiotics in aquaculture and veterinary clinics because of its low side effects and strong bactericidal effect. A total of 45 similar to 60% of florfenicol is not absorbed by the animal body and accumulates in the aquatic environment through a variety of pathways, which affects denitrification. Indoor aquatic microcosm models were constructed and sediment samples were collected at different florfenicol concentrations (0.1, 1, 10, and 100 mg/L) on days 0, 7, 30, and 60 to extract the microbial genome DNA and determine the water properties. qPCR and amplicon sequencing were used to study the dynamic changes in the nirS gene and nirS-type denitrification community structure, diversity, and abundance, respectively. The results showed that high florfenicol stress influenced the sediment's physicochemical properties, reducing conductivity, alkaline dissolved nitrogen, and organic matter content. In addition, the abundance of nirS, a functional denitrification gene, increased obviously with increased florfenicol concentrations but decreased the diversity of nirS-type denitrification microorganisms. Proteobacteria was the dominant denitrifying phylum in the sediment. Our study provides a scientific basis for the rational use of florfenicol in aquaculture to maintain a healthy and stable microecological environment and also provides a preliminary understanding of the response characteristics of water denitrifying microorganisms to florfenicol exposure.
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