文献类型： 外文期刊

作者： Yu, Xiaoxiao ¹ ; Zhang, Zhaoye ³ ; Li, Jianzhen ¹ ; Su, Yingjie ¹ ; Gao, Mingyue ¹ ; Jin, Tingwei ¹ ; Chen, Guang ¹ ;

作者机构： 1.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China

2.Jilin Agr Univ, Minist Educ, Key Lab Straw Biol & Utilizat, Changchun 130118, Peoples R China

3.Jilin Acad Agr Sci, Changchun 130033, Peoples R China

关键词： Co-immobilization enzyme; Cascade reaction; Lignocellulose; Gluconic acid

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2021 年 321 卷

页码：

收录情况： SCI

摘要： The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions.